Summary: protoplasts were not transformable by several different streptomycete plasmids, and was not a host for plaque formation by the bacteriophages ØSE60, ØSE45, ØSE57, ØSE60, ØSE69 or HP10. Extracts of contained DNA-modifying activities that blocked cleavage of plasmid DNA by Nael and , and partially blocked cleavage by Plasmid pOJ434, a derivative of the plasmid pSAS1, was modified by incubation with an extract of to circumvent restriction. Under optimal conditions, protoplasts were transformed to apramycin resistance by modified pOJ434 at frequencies of about 10 per μg of DNA. Transformants contained pOJ434 primarily integrated in the chromosome.


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