1887

Abstract

SUMMARY: Several mutants have been isolated from the facultative methylotroph, , using either -methyl-′-nitro--nitrosoguanidine or ultraviolet light as mutagens. One of these isolates, a glutamate auxotroph lacking isocitrate dehydrogenase, has been transformed to prototrophy, using wild-type DNA, at a frequency of 0·5%. Competence and DNA uptake occur only in cultures which are near the end of exponential growth, and maximal transformation requires a DNA concentration of 100 g ml.

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/content/journal/micro/10.1099/00221287-98-1-265
1977-01-01
2021-08-02
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