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Summary: Escherichia coli k12 cannot grow on d-lyxose, but a mutant was isolated which can utilize d-lyxose as sole source of carbon and energy for growth. d-Lyxose is transported into the bacteria by the d-xylose permease. The mutant constitutively synthesizes a new isomerase which is not inducible in the parent strain under any of the conditions tested. This enzyme, whose native substrate appears to be d-man-nose, fortuitously converts d-lyxose into d-xylulose. Its structural gene is located at around 85 min on the E. coli genetic map, away from other known isomerase genes. d-Xylulose is subsequently catabolized by the enzymes of the normal d-xylose metabolic pathway. d-Mannose isomerase was partially purified and some of its properties were examined.
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