1887

Abstract

Summary: During growth in a liquid culture containing a single soluble or an insoluble cellulosic carbon source, released cellulolytic enzymes into the medium. These enzymes were separated by gel filtration and ion-exchange chromatography into seven components, three of high and four of low molecular weight. One of the components had the character of a C cellulase. When the components were combined they released more reducing sugars from cellulosic substrates than when used singly.

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/content/journal/micro/10.1099/00221287-97-2-251
1976-12-01
2021-05-15
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References

  1. Andrews P. 1964; Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochemical Journal 91:222–233
    [Google Scholar]
  2. Flodin P., Gelotte B., Porath J. 1960; A method for concentrating solutes of high molecular weight. Nature; London: 188493–494
    [Google Scholar]
  3. Li L.H., Flora K.M., King K.W. 1965; Individual roles of cellulase components derived from Trichoderma viride. Archives of Biochemistry and Biophysics 111:439–447
    [Google Scholar]
  4. Lowry O.H., Rdsebrough N.J., Farr A.L., Randall R.J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  5. Miller G.L. 1959; Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31:426–428
    [Google Scholar]
  6. Olutiola P.O. 1972; Aspects of carbohydrate physiology of Rhynchosporium secalis. Ph.D. thesis University of Lancaster;
    [Google Scholar]
  7. Olutiola P.O., Ayres P.G. 1973; A cellulase complex in culture filtrates of Rhynchosporium secalis. Transactions of the British Mycological Society 60:273–282
    [Google Scholar]
  8. Robertson J.S. 1959; Blast disease of the oil palm: its cause, incidence and control in Nigeria. Journal of the West African Institute for Oil Palm Research 2:310–330
    [Google Scholar]
  9. Selby K., Maitland C.C. 1965; The fractionation of Myrothecium verrucaria cellulase by gel-filtration. Biochemical Journal 94:578–583
    [Google Scholar]
  10. Selby K., Maitland C.C. 1967; The cellulase of Trichoderma viride. Separation of the components involved in the solubilization of cotton. Biochemical Journal 104:716–724
    [Google Scholar]
  11. Small W. 1928; The parasitism of Rhizoctonia batalicola (Taub.) Butl. and other fungi. Tropical Agriculturist 20:215–227
    [Google Scholar]
  12. Umeruzike G.M. 1970; Cellulose and β-glucosidase components in culture filtrates from Botryodiplodia theobromae Pat. Journal of Experimental Botany 21:639–650
    [Google Scholar]
  13. Whitaker J.R. 1963; Determination of molecular weights of proteins by gel-filtration on Sephadex. Analytical Chemistry 35:1950–1953
    [Google Scholar]
  14. Whitney P., Chapman J.M., Heale J.B. 1969; Carboxymethylcellulase production by Verticillium albo-atrum. Journal of General Microbiology 56:215–225
    [Google Scholar]
  15. Wood T.M. 1968; Cellulolytic enzyme system of Trichoderma koningii. Separation of components attacking native cotton. Biochemical Journal 109:217–227
    [Google Scholar]
  16. Wootton I.D.P. 1964 Microanalysis in Medical Biochemistry London: Churchill.;
    [Google Scholar]
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