1887

Abstract

SUMMARY: To obtain high yields of extracellular enzymes and toxins for immunological analysis, type culture collection strains of types A to D and 28 fresh isolates of type A from humans were grown in fermenters under controlled conditions in a pre-reduced proteose peptone medium. The type culture collection strains all showed different characteristics with respect to growth rates and pH optima for growth. Production of phospholipase C (alpha-toxin), haemolysin and lethal activity varied considerably between the different types. Growth and extracellular protein production in fermenters with pH control and static or stirred cultures were compared. Production of all extracellular proteins measured was markedly improved by cultivation in fermenters with pH control.

Strain 13124 produced five times more phospholipase C than any of 28 freshly isolated strains of type A, grown under identical conditions. Haemolytic and lethal activities of the ATCC strain were equal or superior to the activities of any of the freshly isolated strains. There were no differences in the bacterial yields and in the production of extracellular toxins between type A strains isolated from clinical cases of gas gangrene and abdominal wounds, and those isolated from faecal samples from healthy persons.

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/content/journal/micro/10.1099/00221287-96-1-137
1976-09-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-96-1-137
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