1887

Abstract

SUMMARY: To obtain high yields of extracellular enzymes and toxins for immunological analysis, type culture collection strains of types A to D and 28 fresh isolates of type A from humans were grown in fermenters under controlled conditions in a pre-reduced proteose peptone medium. The type culture collection strains all showed different characteristics with respect to growth rates and pH optima for growth. Production of phospholipase C (alpha-toxin), haemolysin and lethal activity varied considerably between the different types. Growth and extracellular protein production in fermenters with pH control and static or stirred cultures were compared. Production of all extracellular proteins measured was markedly improved by cultivation in fermenters with pH control.

Strain 13124 produced five times more phospholipase C than any of 28 freshly isolated strains of type A, grown under identical conditions. Haemolytic and lethal activities of the ATCC strain were equal or superior to the activities of any of the freshly isolated strains. There were no differences in the bacterial yields and in the production of extracellular toxins between type A strains isolated from clinical cases of gas gangrene and abdominal wounds, and those isolated from faecal samples from healthy persons.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-96-1-137
1976-09-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/96/1/mic-96-1-137.html?itemId=/content/journal/micro/10.1099/00221287-96-1-137&mimeType=html&fmt=ahah

References

  1. Arvidson S., Holme T., Wadström T. 1971; Influence of cultivation conditions on the production of extracellular proteins byStaphylococcus aureus. . Acta pathologica et microbiologica scandinavica 79B:399–405
    [Google Scholar]
  2. Forget A., Paquette G., Roy A., Fredette V. 1969; Correlations between virulence and other characters ofClostridium perfringens type A. Applied Microbiology 18:668–676
    [Google Scholar]
  3. Gale E. F., Van Heyningen W. E. 1942; The effect of the pH and presence of glucose during growth on the production of α andθ toxins and hyaluronidase byClostridium welchii. . Biochemical Journal 36:624–630
    [Google Scholar]
  4. Goldbarg J. A., Rutenberg A. M. 1965; The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases. Cancer II:283–287
    [Google Scholar]
  5. Hauschild A.H.W. 1966; Selective effect of pH on the production of extracellular protein byClostridium perfringens type D. Journal of Bacteriology 92:800–801
    [Google Scholar]
  6. Hauschild A.H.W. 1971; Clostridium perfringens toxins types B, C, D and E. In Microbial Toxins- BacterialProtein Toxins, 11A: pp. 159–188 Kadis S., Montie T. C., Ajl. S. J. Edited by New York and London:: Academic Press.;
    [Google Scholar]
  7. Holdeman L. V., Moore W.E.C. 1973 Anaerobe Laboratory Manual. Blacksburg, Virginia, U.S.A.:: Virginia Polytechnic Institute and State University.;
    [Google Scholar]
  8. Lynch K. L. 1968; Relationship of route of inoculation and nature of toxin preparation to bioassay ofClostridium perfringens alpha-toxin in mice. Journal of Bacteriology 96:1920–1924
    [Google Scholar]
  9. Möllby R., Wadström T. 1973; Purification of phospholipase C (alpha-toxin) fromClostridium perfringens. . Biochimica et biophysica acta 321:569–584
    [Google Scholar]
  10. Murphey W. H., Barnaby C., Lin F. J., Kaplan N. O. 1967; Malate dehydrogenases. II. Purification and properties ofBacillus subtilis. Bacillus stearothermophilus andEscherichia coli malate dehydrogenases. Journal of Biological Chemistry 242:1548–1559
    [Google Scholar]
  11. Nord C.-E., Möllby R., Smyth C. J., Wadström T. 1974; Formation of phospholipase C and theta- haemolysin in pre-reduced media in hath and continuous culture ofClostridium perfringens type A. Journal of General Microbiology 84:117–127
    [Google Scholar]
  12. Pivnick H., Habeeb A.F.S.A., Gorenstein B. S., Stuart P. F., Hauschild A.H.W. 1964; Effect of pH on toxinogenesis byClostridium perfringens type C. Canadian Journal of Microbiology 10:329–344
    [Google Scholar]
  13. Raynaud M., Turpin A., Mangalo R., Bizzini B. 1955; Croissance et toxinogenese. Annales de rinstitut Pasteur 88:24–44
    [Google Scholar]
  14. Sargeant K. 1968; Improvement of yields in anaerobic cultures. Chemistry and Industry, no. 85–88
    [Google Scholar]
  15. Smith L.D.S. 1973; The Clostridia. In Handbook of Microbiology. I, Organismic Microbiology, pp. 89–96 Laskin A. I., Lechevalier. A. I. Edited by Cleveland, Ohio, U.S.A.:: Chemical Rubber Co. Press.;
    [Google Scholar]
  16. Smith L.D.S., Holdemaa L.V. 1968 The Pathogenic Anaerobic Clostridia. Springfield, Illinois, U.S.A.:: Charles C. Thomas.;
    [Google Scholar]
  17. Weeke B. 1973; Crossed Immunoelectrophoresis. Scandinavian Journal of Immunology (Suppl. 1/73) 2:47–69
    [Google Scholar]
  18. Willis A. T. 1969 Clostridia of Wound Infection. London:: Butterworths.;
    [Google Scholar]
  19. Wilsdon A. J. 1931 Reports of the Institute for Pathology, University of Cambridge, 2nd report pp. 53–85
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-96-1-137
Loading
/content/journal/micro/10.1099/00221287-96-1-137
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error