SUMMARY: The apparent ATP requirement for N fixation in was high (the ATP/N molar ratio was 29 when estimated in anaerobic glucose-limited chemostat cultures) compared with that determined previously in and in The high value was probably not due to unfavourable temperature, phosphate concentration or pH. The apparent ATP requirement for N fixation was probably no lower in O-limited chemostat cultures than in anaerobic glucose-limited chemostat cultures. When hydrogen was introduced into the atmosphere over the anaerobic glucose-limited chemostat culture, there was an increase in the apparent ATP requirement for N fixation and in the activity of nitrogenase in intact organisms. A comparison of these increases suggests that some ATP is wasted by the ATP-dependent H-evolving activity of nitrogenase.


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