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SUMMARY: A morphological mutant of Paracoccidioides brasiliensis strain ivic pb9 was isolated after treatment of the yeast-like (Y) form with nitrosoguanidine. Colonies of the mutant grown at room temperature did not show the whitish cotton-like morphology typical of the mycelial form of the parental strain. Y-cells were much smaller than those produced by the parent and grew forming chains of different sizes. The main chemical difference in the wall of the Y-form was the replacement of the α-1,3-glucan, typical of the parental strain, by an amorphous 1,3-mannan in the mutant.
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