1887

Abstract

SUMMARY: Amino acids liberated by peptidase hydrolysis of di-and oligopeptides by were measured by trinitrobenzenesulphonate assay and high voltage electrophoresis or paper chromatography followed by ninhydrin spray. Intact bacteria or periplasmic contents released by lysozyme treatment did not hydrolyse peptides. Subcellular fractionation showed that glycylmethionine peptidase activity was cytoplasmic. This enzyme had a of 2 m, and was stimulated fivefold by 1 m-Co. Crude peptidase extract did not cleave peptides with -residues, acylated N-terminal amino groups or -methylated peptide bonds but otherwise showed a wide specificity. Di-or tripeptides with blocked C-terminus were hydro-lysed. Leucylleucine (12 m) and leucylglycylglycine (10 m) did not compete with glycylmethionine (1·m) and glycylmethionylglycine (1·0 m), respectively, for hydrolysis. also contained peptidase activity (0·84 mol amino acid released from glycylmethionylglycine/min/mg protein). Peptidases of both and were constitutive.

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/content/journal/micro/10.1099/00221287-92-2-296
1976-02-01
2021-10-26
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References

  1. Becker J. M., Naider F., Katchalski E. 1973; Peptide utilization in yeast. Studies of methionine and lysine auxotrophs of Saccharomyces cerevisiae. Biochemica et biophysica acta 291:388–397
    [Google Scholar]
  2. Behal F. J., Folds J. D. 1967; A comparative study of bacterial alanine aminohydrolases. Biochemical and Biophysical Research Communications 27:344–349
    [Google Scholar]
  3. Binkley F., Leibach F., King N. 1968; A new method of peptidase assay and the separation of three leucylglycinases of renal tissues. Archives of Biochemistry and Biophysics 128:397–405
    [Google Scholar]
  4. Brenner M., Huber W. 1953; Herstellung von α-Aminosäureestern durch Alkoholyse der Methylester. Helvetica chimica acta 36:1109–1115
    [Google Scholar]
  5. Cascieri T., Mallette M. F. 1976; Peptide utilization by Pseudomonas putida and Pseudomonas maltophilia. Journal of General Microbiology 92:283–295
    [Google Scholar]
  6. Goldberg A. L., Dice J. F. 1974; Intracellular protein degradation in mammalian and bacterial cells. Annual Review of Biochemistry 43:835–869
    [Google Scholar]
  7. Hauge J. G. 1966; Glucose dehydrogenases-particulate. I.Pseudomonas species and Bacterium anitratum. Methods in Enzymology 9:92–98
    [Google Scholar]
  8. Hylemon P. B., Phibbs P. V. 1972; Independent regulation of hexose catabolizing enzymes and glucose transport activity in Pseudomonas aeruginosa. Biochemical and Biophysical Research Communications 48:1041–1048
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Miller C. G., Mackinnon K. 1974; Peptidase mutants of Salmonella typhimurium. Journal of Bacteriology 120:355–363
    [Google Scholar]
  11. Naider F., Becker J. M., Katzir-Katchalski E. 1974; Utilization of methionine-containing peptide and their derivatives by a methionine-requiring auxotroph of Saccharomyces cerevisiae. Journal of Biological Chemistry 249:9–20
    [Google Scholar]
  12. Payne j. W. 1968; Oligopeptide transport in Escherichia coli. Specificity with respect to side chain and distinction from dipeptide transport. Journal of Biological Chemistry 243:3395–3403
    [Google Scholar]
  13. Payne J. W. 1972; The characterization of dipeptidases from Escherichia coli. Journal of General Microbiology 71:267–279
    [Google Scholar]
  14. Payne J. W., Gilvarg C. 1971; Peptide transport. Advances in Enzymology 35:187–244
    [Google Scholar]
  15. Pine M. J. 1972; Turnover in intracellular proteins. Annual Review of Microbiology 26:103–126
    [Google Scholar]
  16. Simmonds S., Toye N. O. 1966; Peptidases in spheroplasts of Escherichia coli K-12. Journal of Biological Chemistry 241:3852–3860
    [Google Scholar]
  17. Stinnett J. D., Guymon L. F., Eagon R. G. 1973; A novel technique for the preparation of transport-active membrane vesicles from Pseudomonas aeruginosa: observations on gluconate transport. Biochemical and Biophysical Research Communications 52:284–290
    [Google Scholar]
  18. Wolfinbarger L., Marzluf G. A. 1974; Peptide utilization by amino acid auxotrophs of Neurospora crassa. Journal of Bacteriology 119:371–378
    [Google Scholar]
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