1887

Abstract

Summary: The activities of polymyxin B sulphate, colistin (polymyxin E) sulphate and their sulphomethyl derivatives were compared by continuous turbidimetric monitoring of dense cultures of an strain exposed to these agents. Judged by the concentration of antibiotic which caused a rapid fall in opacity of the culture, polymyxin B sulphate and colistin sulphate had similar activities, but the sulphomethyl compounds differed considerably: sulphomyxin sodium induced lysis of the culture at a concentration four times that of the parent compound, whereas colistin sulphomethate sodium induced a delayed fall in opacity consistent with recruitment of activity as the inactive sulphomethyl derivative was broken down to the parent compound.

During overnight incubation, regrowth of cultures which had initially succumbed to polymyxin action occurred, apparently due to the selection of phenotypically resistant variants from within the population. In this way cultures could easily be adapted to growth in concentrations of antibiotic well above the conventionally-determined minimum inhibitory concentration. The comparative ease of adaptation was in the order: colistin sulphomethate > sulphomyxin > colistin sulphate > polymyxin B sulphate.

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1975-11-01
2021-10-18
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References

  1. Barnett M., Bushby S. R. M., Wilkinson S. 1964; Sodium sulphomethyl derivatives of polymyxins. British Journal of Pharmacology 23:552–574
    [Google Scholar]
  2. Benveniste R., Davies J. 1973; Mechanisms of antibiotic resistance in bacteria. Annual Review of Biochemistry 42:471–506
    [Google Scholar]
  3. Beveridge E. G., Martin A. J. 1967; Sodium sulphomethyl derivatives of polymyxins. British Journal of Pharmacology and Chemotherapy 29:125–135
    [Google Scholar]
  4. Bushby S. R. M. 1955; Sensitivity tests for the polymyxins. Journal of Clinical Pathology 8:120–122
    [Google Scholar]
  5. Dunnick J.K, O’Leary W.M. 1970; Correlation of bacterial lipid composition with antibiotic resistance. Journal of Bacteriology 101:892–900
    [Google Scholar]
  6. Gale E. F., Cundliffe E., Reynolds P. E., Richmond M. H., Waring M. J. 1972 The Molecular Basis of Antibiotic Action. London:: John Wiley.;
    [Google Scholar]
  7. Greenwood D., O’Grady F. 1969; Antibotic-induced surface changes in microorganisms demonstrated by scanning electron microscopy. Science; New York: 1631076–1078
    [Google Scholar]
  8. Greenwood D., O’Grady F. 1973; Comparison of the responses of Escherichia coli and Proteus mira-bilis to seven beta-lactam antibiotics. Journal of Infectious Diseases 128:211–222
    [Google Scholar]
  9. Jawetz E., Coleman V. R. 1949; Laboratory and clinical observations on Aerosporin (polymyxin B). Journal of Laboratory and Clinical Medicine 34:751–760
    [Google Scholar]
  10. Kauffmann J. 1974; Méchanisme de l’effet inoculum (effet de population) chez Pseudomonas aeruginosa sur milieu additionné de polymyxine. Comptes rendus hebdomadaire des seances de I’Académie des sciences 279D:207–209
    [Google Scholar]
  11. Mackintosh I. P., O’Grady F., Greenwood D., Watson B. W., Crichton T. C., Piper R., Ferrer A. 1973; A twelve channel bacterial growth monitoring system. Biomedical Engineering 8:514–515 526
    [Google Scholar]
  12. Marr A. G., Ingraham J. L. 1962; Effect of temperature on the composition of fatty-acids in Escherichia coli. Journal of Bacteriology 84:1260–1267
    [Google Scholar]
  13. McCabe W. R., Jackson G. G., Kozij V. M. 1960; Clinical and laboratory observations on the use of colistin in infections by Gram-negative bacilli. In Antibiotics Annual 1959 1960 pp. 80–88 Marti-Ibanez F. New York:: Antibiotica.;
    [Google Scholar]
  14. Schwartz B. S. 1964; The polypeptides of the polymyxin group. In Experimental Chemotherapy 3 pp. 217–270 Schnitzer R.H, Hawking F. New York:: Academic Press.;
    [Google Scholar]
  15. Sebek O. K. 1967; Polymyxins and circulin. In Antibiotics 1 Mode of Action pp. 142–152 Gottlieb D., Shaw P.D. Berlin:: Springer Verlag.;
    [Google Scholar]
  16. Yudkin J. 1953; Origin of acquired drug resistance in bacteria. Nature; London: 171541–546
    [Google Scholar]
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