1887

Abstract

SUMMARY: A new method is described for the efficient conversion of cells into protoplasts. The following parameters of guanine uptake determined in whole cells were unchanged in protoplasts: value, requirement for an energy source, sensitivity to competitive inhibitors, pH optimum, as well as the typical variation of the initial velocity of uptake observed during the growth phase.

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/content/journal/micro/10.1099/00221287-90-2-260
1975-10-01
2021-08-03
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References

  1. Bacon J. S. D., Jones D., Farmer V. C., Webley D. M. 1968; The occurrence of α-(1→3) glucan in Cryptococcus, Schizosaccharomyces and polyporus species, and its hydrolysis by a streptomyces culture filtrate lysing cell walls of Cryptococcus. Biochimica et biophysica acta 158:313–315
    [Google Scholar]
  2. Bush D. A., Horisberger M., Horman I., Wüsch P. 1974; The wall structure of Schizosaccharomyces pombe. Journal of General Microbiology 81:199–206
    [Google Scholar]
  3. Cummins J. E., Mitchison J. M. 1967; Adenine uptake and pool formation in the fission yeast Schizosaccharomyces pombe. Biochimica et biophysica acta 136:108–120
    [Google Scholar]
  4. Fleet G. H., Phaff H. J. 1973; Effects of glucanases of yeast and bacterial origin on cell walls of Schizosaccharomyces species in yeast. In Mould and Plant Protoplasts pp. 33–58 New York: Academic Press.;
    [Google Scholar]
  5. Foury F., Goffeau A. 1973; Combination of 2-deoxyglucose and snail-gut enzyme treatments for preparing sphaeroplasts of Schizosaccharomyces pombe. Journal of General Microbiology 75:227–229
    [Google Scholar]
  6. Heppel L. A. 1971; The concept of periplasmic enzymes. In The Structure and Function of Biological Membranes pp. 224–245 Rothfield L. I. Edited by New York: Academic Press.;
    [Google Scholar]
  7. Mitchison J. M., Creanor J., Sartirana M. L. 1973; Enzyme synthesis, RNA synthesis and the effects of actinomycin D in protoplasts of the fission yeast Schizosaccharomyces pombe. Proceedings of the Third International Symposium in Yeast Protoplasts Salamanca, Spain pp. 229–245 London: Academic Press.;
    [Google Scholar]
  8. Phaff H. J. 1971; Structure and biosynthesis of the cell yeast envelope. In The Yeasts 2 pp. 135–202 Rose A. H., Harrison J. S. Edited by New York: Academic Press.;
    [Google Scholar]
  9. Pourquié J. 1970; Antagonism by adenine in the nutrition of Schizosaccharomyces pombe mutants. Inhibition at the level of guanine uptake. Biochimica et biophysica acta 209:269–277
    [Google Scholar]
  10. Svihla G., Schlenk F., Dainko J. L. 1961; Spheroplasts of the yeast Candida utilis. Journal of Bacteriology 82:808–814
    [Google Scholar]
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