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SUMMARY: In order to prepare staphylococcal cell walls as free as possible from intact organisms yet with minimum chemical and mechanical degradation, a rapid turbidimetric method was developed to measure the proportion of staphylococci ruptured by shaking with glass beads. The assay took less than a minute, and was used to find the bacterial concentration and speed and time of shaking which gave the biggest yields of cell walls in the shortest time.
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