@article{mbs:/content/journal/micro/10.1099/00221287-9-2-199, author = "Cooper, P. D.", title = "The Study of Cell Rupture in Staphylococcus aureus", journal= "Microbiology", year = "1953", volume = "9", number = "2", pages = "199-206", doi = "https://doi.org/10.1099/00221287-9-2-199", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-9-2-199", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: In order to prepare staphylococcal cell walls as free as possible from intact organisms yet with minimum chemical and mechanical degradation, a rapid turbidimetric method was developed to measure the proportion of staphylococci ruptured by shaking with glass beads. The assay took less than a minute, and was used to find the bacterial concentration and speed and time of shaking which gave the biggest yields of cell walls in the shortest time.", }