1887

Abstract

SUMMARY: The dissimilatory sulphate-reducing bacterium frequently sub-cultured, often contained spherical granules which stained metachromatically with some basic dyes. The granules were examined by transmission electron microscopy of whole organisms and thin sections. The granules were isolated from broken bacteria as a water-insoluble, non-crystalline, white material containing magnesium, phosphorus and organic carbon, but devoid of sulphur and nitrogen. The molar ratio of phosphorus to magnesium (1·17) was close to the proportions in magnesium tripolyphosphate. Infrared absorption spectra for the white material and magnesium tripolyphosphate were similar.

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1975-07-01
2021-07-29
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References

  1. Ashby G. K. 1938; Simplified Schaeffer spore stain. Science; New York: 87443
    [Google Scholar]
  2. Burdon K. L. 1946; Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. Journal of Bacteriology 52:665–678
    [Google Scholar]
  3. Chen P. S. JUN. Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus. Analytical Chemistry 28:1756–1758
    [Google Scholar]
  4. Corbridge D. E. C., Lowe E. J. 1954; The infra-red spectra of inorganic phosphorus compounds. Journal of the Chemical Society493–502
    [Google Scholar]
  5. Corbridge D. E. C., Lowe E. J. 1955; Quantitative infrared analysis of condensed phosphates. Analytical Chemistry 271383–1387
    [Google Scholar]
  6. Cruickshank R. 1965 Medical Microbiology, 11th edn.. Edinburgh and London: E. and S. Livingstone;
    [Google Scholar]
  7. Doudoroff M., Stanier R. Y. 1959; Role of poly-β-hydroxybutyric acid in the assimilation of organic carbon by bacteria. Nature; London: 1831440–1442
    [Google Scholar]
  8. Forsyth W. G. C., Hayward A. C., Roberts J. B. 1958; Occurrence of poly-β-hydroxybutyric acid in aerobic Gram-negative bacteria. Nature; London: 182800–801
    [Google Scholar]
  9. Harold F. M. 1966; Inorganic polyphosphates in biology: structure, metabolism, and function. Bacteriological Reviews 30:772–794
    [Google Scholar]
  10. Harold F. M., Sylvan S. 1963; Accumulation of inorganic polyphosphate in Aerobacter aerogenes. II. Environmental control and the role of sulfur compounds. Journal of Bacteriology 86:222–231
    [Google Scholar]
  11. Hotchkiss R. D. 1948; A microchemical reaction resulting in the staining of polysaccharide structures in fixed tissue preparations. Archives of Biochemistry 16:131–141
    [Google Scholar]
  12. Jones H. E. 1971; Sulfate-reducing bacterium with unusual morphology and pigment content. Journal of Bacteriology 106:339–346
    [Google Scholar]
  13. Jones H. E., Skyring G. W. 1974; Reduction of sulphite to sulphide catalysed by desulfoviridin from Desulfovibrio gigas. Australian Journal of Biological Sciences 27:7–14
    [Google Scholar]
  14. Kellenberger E., Ryter A., Séchaud J. 1958; Electron microscope study of DNA-containing plasma. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. Journal of Biophysical and Biochemical Cytology 4:671–682
    [Google Scholar]
  15. King E. J. 1951 Micro Analysis in Medical Biochemistry London: J. and A. Churchill;
    [Google Scholar]
  16. Law J. H., Slepecky R. A. 1961; Assay of poly-β-hydroxybutyric acid. Journal of Bacteriology 82:33–36
    [Google Scholar]
  17. Lemoigne M. 1927; Études sur l’autolyse microbienne origine de l’acide β-oxybutyrique forme par autolyse. Annales de l’Institut Pasteur 41:148–165
    [Google Scholar]
  18. Macrae R. M., Wilkinson J. F. 1958; Poly-β-hydroxybutyrate metabolism in washed suspensions of Bacillus cereus and Bacillus megaterium. Journal of General Microbiology 19:210–222
    [Google Scholar]
  19. Martinez R. J. 1963; On the nature of the granules of the genus Spirillum. Archiv für Mikrobiologie 44:334–343
    [Google Scholar]
  20. Miller F. A., Wilkins C. H. 1952; Infrared spectra and characteristic frequencies of inorganic ions. Analytical Chemistry 24:1253–1294
    [Google Scholar]
  21. Mulder E. G., Van Veen W. L. 1963; Investigations of the Sphaerotilus-Leptothrix group. Antonie van Leeuwenhoek 2:121–153
    [Google Scholar]
  22. Reichelt J. L., Baumann P. 1973; Taxonomy of the marine, luminous bacteria. Archiv für Mikro-biologie 94:283–330
    [Google Scholar]
  23. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cellular Biology 17:208–212
    [Google Scholar]
  24. Sicko L. M. 1972; Structural variations of polyphosphate bodies in blue-green algae. Proceedings of the Electron Microscopy Society of America 30:218–219
    [Google Scholar]
  25. Spurr A. R. 1969; A low viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26:31–43
    [Google Scholar]
  26. Thilo E. 1962; Condensed phosphates and arsenates. Advances in Inorganic Chemistry and Radiochemistry 4:1–77
    [Google Scholar]
  27. Tobback P., Laudelout H. 1965; Poly-β-hydroxybutyric acid in Nitrobacter. Biochimica et biophysica acta 97:589–590
    [Google Scholar]
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