SUMMARY: Six auxotrophic markers of a halotolerant collagenolytic strain of Achromobacter were transduced by four α phages. Abortive transduction was also demonstrated. The generalized transduction system is unusual as the transductants were unstable, characteristic of transduction by lysogeny. The Achromobacter strain is a cryptic lysogen for α and purified transductants were either sensitive or resistant to α. Purified clones from four resistant transductants and one sensitive transductant liberated phage spontaneously. The host ranges of these spontaneous phages differed from that of the α phage used for the transduction experiment. Some initially resistant transductants became semi-sensitive to α (efficiency of plating [e.o.p.] 10 to 10) after repeated cloning.


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