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SUMMARY: Polyacrylamide gel electrophoresis and isoelectric focusing techniques have been used to compare NAD-dependent l(+)lactate dehydrogenases (LDH) from ten different strains of Mycoplasma mycoides var. mycoides. The enzymes were not distinguished from one another, or from normal bovine LDH 1 by these methods.
The kinetic behaviour of LDH from M. mycoides (t 1 vaccine strain) suggested that the enzyme could readily reduce pyruvate or oxidize lactate in a manner which, in vertebrates, requires two different isoenzymes.
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