1887

Abstract

Summary: -Glutamate inhibited hyphal extension, the degree of inhibition depending on the identity of the glutamate dehydrogenase (GDH) enzyme present in the mycelium. Mycelia were most sensitive to inhibition on media which promoted formation of the NAD-linked GDH. The activity of this enzyme was inhibited by -glutamate in experiments with cell-free extracts, the inhibitions being noncompetitive or mixed. Similarly the activity of the NADP-linked enzyme (GDH) was inhibited in its utilization of ammonium ions (in the amination reaction) and -glutamate (in the deamination reaction). In contrast, 2-oxoglutarate was a co-operative substrate for GDH in the amination reaction and -glutamate acted as an allosteric activator. -Glutamate was also able to derepress the synthesis of GDH; the NAD-linked enzyme was hardly affected.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-85-2-274
1974-12-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/85/2/mic-85-2-274.html?itemId=/content/journal/micro/10.1099/00221287-85-2-274&mimeType=html&fmt=ahah

References

  1. Arkin H., Grossowicz N. 1970; Inhibition by D-glutamate of growth and glutamate dehydrogenase activity of Neurospora crassa. Journal of General Microbiology 61:255–261
    [Google Scholar]
  2. Dennen D. W., Niederpruem D. J. 1965; Control of glutamate dehydrogenase in the Basidiomycete Schizophyllum commune. Life Sciences 4:93–98
    [Google Scholar]
  3. Fawole M. O., Casselton P. J. 1972; Observations on the regulation of glutamate dehydrogenase activity in Coprinus lagopus. Journal of Experimental Botany 23:530–551
    [Google Scholar]
  4. Frieden C. 1963; Glutamate dehydrogenase. IV. Studies on enzyme inactivation and coenzyme binding. Journal of Biological Chemistry 238:146–154
    [Google Scholar]
  5. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  6. Monod J., Wyman J., Changeux J. P. 1965; On the nature of allosteric transition. A plausible model. Journal of Molecular Biology 12:88–118
    [Google Scholar]
  7. Moore D. 1968; The effect of 2-deoxy-D-glucose on the growth and respiration of Coprinus lagopus. Journal of General Microbiology 52:433–439
    [Google Scholar]
  8. Moore D., Stewart G. R. 1972; Effects of 2-deoxy-D-glucose, D-glucosamine, and L-sorbose on the growth of Coprinus lagopus hyphae. Journal of General Microbiology 71:333–342
    [Google Scholar]
  9. Olson J. A., Anfinsen C. B. 1953; Kinetic and equilibrium studies on crystalline L-glutamic acid dehydrogenase. Journal of Biological Chemistry 202:841–856
    [Google Scholar]
  10. Roberts E. 1953; Further studies of inhibition of bacterial glutamic decarboxylase. Journal of Biological Chemistry 202:359–367
    [Google Scholar]
  11. Sanwal B. D., Lata M. 1962; Concurrent regulation of glutamic acid dehydrogenases of Neurospora. Archives of Biochemistry and Biophysics 97:582–588
    [Google Scholar]
  12. Stewart G. R., Moore D. 1974; The activities of glutamate dehydrogenases during mycelial growth and sporophore development in Coprinus lagopus {sensu Lewis). Journal of General Microbiology 83:73–81
    [Google Scholar]
  13. Stine G. J. 1968; Enzyme activities during the asexual cycle of Neurospora crassa. II. NAD- and NADP-dependent glutamic dehydrogenases and nicotinamide adenine dinucleotidase. Journal of Cell Biology 37:81–88
    [Google Scholar]
  14. Tuveson R. W., West D. J., Barratt R. W. 1967; Glutamic acid dehydrogenases in quiescent and germinating conidia of Neurospora crassa. Journal of General Microbiology 48:235–248
    [Google Scholar]
  15. Webb J. L. 1963 Enzyme and Metabolic Inhibitors vol. 1 New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-85-2-274
Loading
/content/journal/micro/10.1099/00221287-85-2-274
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error