The Genetic Analysis of Resistance to Benomyl in Neurospora crassa

SUMMARY: Mutants of Neurospora crassa resistant to the fungicide, methyl-1-(butyl-carbamoyl)-benzimidazolecarbamate, were isolated following u.v. irradiation of conidia. Genetic analysis of 15 of the mutant strains revealed that resistance was due to a single allele averaging 2·9 map units right of ylo-1, 17·9 units right of tryp-2 and 32·1 units left of chol-2 in linkage group VI. Tests of heterokaryons containing both resistant and sensitive alleles indicated that resistance is dominant. The mutants did not possess the same degree of tolerance to the fungicide.