1887

Abstract

SUMMARY: Fourteen of the sixteen nucleotides present in extracts of were identified by thin-layer chromatography. Data are presented describing the incorporation of radioactive bases into the guanine nucleotide pools and into RNA. Studies with labelled guanine suggested that a small fraction of the RNA undergoes rapid turnover at the pre-culmination stage of differentiation. Analytical methods are described for determining flux rates and the percentage turnover of endogenous pools.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-84-1-173
1974-09-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/84/1/mic-84-1-173.html?itemId=/content/journal/micro/10.1099/00221287-84-1-173&mimeType=html&fmt=ahah

References

  1. Barravecchio J., Baumann P., Wright B. E. 1969; Cell volume determinations of Dictyostelium discoideum . Journal of Applied Microbiology 17:641–644
    [Google Scholar]
  2. Bonner J. T. 1947; Evidence for the formation of cell aggregates by chemotaxis in the development of the slime mold Dictyostelium discoideum . Journal of Experimental Zoology 106:1–26
    [Google Scholar]
  3. Ceccarini C., Filosa M. 1965; Carbohydrate content during development of the slime mold, Dictyostelium discoideum . Journal of Cellular and Comparative Physiology 66:135–139
    [Google Scholar]
  4. Cowie D. B., Bolton E. 1957; The use of metabolic pools of purine compounds for nucleic acid synthesis in yeast. Biochimica et biophysica acta 25:292–298
    [Google Scholar]
  5. Firtel R. A., Lodish H. F. 1973; A small nuclear precursor of messenger RNA in the cellular slime mold Dictyostelium discoideum . Journal of Molecular Biology 79:295–314
    [Google Scholar]
  6. Gustafson G. L., Wright B. E. 1972; Analysis of approaches used in studying differentiation of the cellular slime mold. In Critical Reviews of Microbiology vol. 1 pp. 453–478 Edited by Laskin A. I., Lechevalier H. Cleveland, Ohio: CRC Press;
    [Google Scholar]
  7. Hirschberg E., Ceccarini C., Osnos M., Carchman R. 1968; Effects of inhibitors of nucleic acid, and protein synthesis on growth and aggregation of the cellular slime mold. Dictyostelium discoideum Proceedings of the National Academy of Sciences of the United States of America 61:316–323
    [Google Scholar]
  8. Hurlbert R. B., Hardy D. 1972; Turnover of Hn RNA and PolyA in rat liver nuclei. Federation Proceedings 31:428–430
    [Google Scholar]
  9. Inselburg J., Sussman M. 1967; Incorporation of 3H-uridine into RNA during cellular slime mould development. Journal of General Microbiology 46:59–64
    [Google Scholar]
  10. Kessin R. H. 1973; RNA metabolism during vegetative growth and morphogenesis of the cellular slime mold Dictyostelium discoideum . Developmental Biology 31:242–251
    [Google Scholar]
  11. Kijima S., Wilt F. H. 1969; Rate of nuclear ribonucleic acid turnover in sea urchin embryos. Journal of Molecular Biology 40:235–246
    [Google Scholar]
  12. Leighton T. J., Doi R. H. 1971; The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis . Journal of Biological Chemistry 246:3189–3195
    [Google Scholar]
  13. Marshall R., Sargent D., Wright B. E. 1970; Glycogen turnover in Dictyostelium discoideum . Biochemistry 9:3087–3094
    [Google Scholar]
  14. Newell P. C., Telser A., Sussman M. 1969; Alternative developmental pathways determined by environmental conditions in the cellular slime mold Dictyostelium discoideum . Journal of Bacteriology 100:763–768
    [Google Scholar]
  15. Nierlich D. P., Vielmetter W. 1968; Kinetic studies on the relationship of ribonucleotide precursor pools and ribonucleic acid synthesis. Journal of Molecular Biology 32:135–147
    [Google Scholar]
  16. Pannbacker R. G. 1966; RNA metabolism during differentiation in the cellular slime mold. Biochemical and Biophysical Research Communications 24:340–345
    [Google Scholar]
  17. Pannbacker R. G. 1967; Uridine diphosphate biosynthesis during differentiation in the cellular slime mold. I. In vivo measurements. Biochemistry 6:1283–1286
    [Google Scholar]
  18. Plagemann P. G. W. 1971; Nucleotide pools of NovikofF hepatoma cells growing in suspension culture. II Independent nucleotide pools for nucleic acid synthesis. Journal of Cellular Physiology 77:241–258
    [Google Scholar]
  19. Plagemann P. G. W. 1972; Nucleotide pools of NovilofF hepatoma cells growing in suspension culture. III Effects of nucleosides in medium on levels of nucleotides in separate nucleotide pools for nuclear and cytoplasmic RNA synthesis. Journal of Cellular Biology 52:131–146
    [Google Scholar]
  20. Randerath E., Randerath K. 1964a; Resolution of complex nucleotide mixtures by two dimensional anion-exchange thin-layer chromatography. Journal of Chromatography 16:126–129
    [Google Scholar]
  21. Randerath E., Randerath K. 1965; Ion-exchange thin-layer chromatography. XII. Quantitative elution and microdetermination of nucleoside monophosphate, ATP, and other nucleotide coenzymes. Analytical Biochemistry 12:89–93
    [Google Scholar]
  22. Randerath K., Randerath E. 1964b; Ion exchange chromatography of nucleotides on poly(ethyleneimine)-cellulose thin layers. Journal of Chromatography 16:111–125
    [Google Scholar]
  23. Roberts R., Abelson P., Cowie D., Boulton E., Britten P. 1963; Annual Report of the Director of the Department of Terrestrial Magnetism. Carnegie Institute, Washington, D.C 607:190–196
    [Google Scholar]
  24. Rutherford C. L., Wright B. E. 1971; Nucleotide metabolism during differentiation in Dictyostelium discoideum . Journal of Bacteriology 108:269–275
    [Google Scholar]
  25. Salser W., Janin J., Levinthal C. 1968; Measurement of the unstable RNA in exponentially growing cultures of Bacillus subtilis and Escherichia coli . Journal of Molecular Biology 31:237–266
    [Google Scholar]
  26. Sargent D., Wright B. E. 1971; Trehalose synthesis during differentiation in Dictyostelium discoideum.II. In vivo flux determinations. Journal of Biological Chemistry 246:5340–5344
    [Google Scholar]
  27. Watts J. W. 1964; Turnover of nucleic acids in a multiplying animal cell. Biochemical Journal 93:306–312
    [Google Scholar]
  28. Weber M. J., Rubin H. 1970; Uridine transport and RNA synthesis in growing and in density-inhibited animal cells. Journal of Cell Physiology 77:157–168
    [Google Scholar]
  29. Wright B. E. 1968; An analysis of metabolism underlying differentiation in Dictyostelium discoideum. Annual Conference on Molecular Aspects of Differentiation, Oak Ridge National Laboratory. Journal of Cell Physiology 72:SI145–160
    [Google Scholar]
  30. Wright B. E., Anderson M. L. 1960; Protein and amino acid turnover during differentiation in the slime mold. II. Incorporation of 35S methionine into the amino acid pool and into protein. Biochimica et biophysica acta 43:67–78
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-84-1-173
Loading
/content/journal/micro/10.1099/00221287-84-1-173
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error