SUMMARY: The uptake and synthesis of folate polyglutamates from [H]pteroylglutamic acid and [C]-aminobenzoic acid was studied in and . The analytical techniques were based on oxidative or hydrolytic degradation of the folate compounds followed by chromatographic separation of the resulting mixtures of pteroylglutamate derivatives or -aminobenzoylpoly-γ--glutamates and comparison with appropriate synthetic standards. All four organisms synthesized numerous polyglutamate derivatives from the precursors provided. The large majority (85 to 95%) of folates of lactobacilli were polyglutamates with more than seven glutamyl residues; formed several such unidentified long-chain components. In contrast, and particularly synthesized mainly polyglutamates with seven or less glutamate residues; tetra-, hexa- and heptaglutamates were tentatively identified in , and tri-, tetra- and pentaglutamates in . While appeared to synthesize a mixture of formyl- and methyltetrahydro derivatives, formyltetrahydro derivatives predominated in and .


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