1887

Abstract

SUMMARY: A method for the selection of auxotrophic mutants in using -glycosyl-polifungin has been developed. The method results in about 9000-fold enrichment. All types of auxotrophs were obtained. Under certain conditions preferential selection of nucleic acid hydrolysate-requiring auxotrophs was observed. The frequency of occurrence of these mutants was about 50% of all colonies tested. Cysteine auxotrophs of were obtained for the first time by means of this method.

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/content/journal/micro/10.1099/00221287-84-1-111
1974-09-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-84-1-111
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