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Summary: Membrane vesicles of Thiobacillus neapolitanus take up amino acids at 25 °C in the presence of the nonphysiological electron donor, ascorbate- N,N,N′,N′-tetramethyl- p -phenylenediamine. The amino acids accumulate inside the membrane vesicles against a concentration gradient. Inhibitors of the electron transport chain inhibit the accumulation; therefore active transport of amino acids in T. neapolitanus is coupled to the electron transport chain. The Km values for the transport of glycine and l-serine in this organism are 2·5 and 5 μ m respectively.
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