SUMMARY: Protoplasts of cultivaued in sporulation medium with an osmotic stabilizer at pH 7.0 were studied by means of light and electron microscopy. After 15 h, mature ascospores with complese cell walls were formed in the protoplasts. During sporulation, protoplasts regenerated only the fibrillar wall component and were osmotically sensitive. By using snail enzymes to block fibril regeneration on the protoplast surface, it was proved that sporulation was quite independent of such regeneration. Most of the ascospores produced by protoplasts were viable, osmotically resistant and save rise to new yeast cells. Meiotic division and sporulation in the yeasts are processes quite independent of the presence of the ascus wall.


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