Skip to content
1887

Microbiology Society logo Microbiology

Volume 82, Issue 1

Effect of Potassium Cyanide, Glucose and Anaerobiosis on Morphogenesis of Free

Abstract

Summary: The morphological effect of potassium cyanide on the aerobic germination of spores of (1894) in the presence of glucose was investigated. At concentrations of up to 2 m-KCN, normal filaments were formed; at concentrations of 3 to 6 m intermediate forms were observed ranging from enlarged and septated hyphae to rounded multipolar cells; at 8 m spores produced only budding spherical cells. The effect of glucose on the anaerobic germination of spores was also studied. At 0·01 % glucose hyphal development occurred, whereas at increasing concentrations dimorphic colonies were formed. At 2 % glucose purely yeast colonies appeared of the type described by Bartnicki-García (1968) for 8o. In the absence of glucose, or when glucose was replaced by xylose, maltose or succinate, spores did not germinate.

Anaerobiosis did not prevent the elongation of preformed hyphae obtained under aerobic conditions, provided that the cellular integrity was maintained. The anaerobic mycelia had a fermentative metabolism, as indicated by the low level of cytochrome oxidase, the amount and the type of pyruvate kinase, and the production of ethyl alcohol. Conversion of hyphal to yeast-like morphology was observed when tips of young hyphae were cultured in conditions favouring fermentation. The budding cells obtained were indistinguishable from yeast-like cells generated by anaerobic germination of spores.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology 1974
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-82-1-15
1974-05-01
2025-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/82/1/mic-82-1-15.html?itemId=/content/journal/micro/10.1099/00221287-82-1-15&mimeType=html&fmt=ahah

References

  1. Bartnicki-García S. 1968; Control of dimorphism in Mucor by hexoses: inhibition of hyphal morphogenesis. Journal of Bacteriology 96:1586–1594
     [Google Scholar]
  2. Bartnicki-García S., & Nickerson W. J. 1962a; Induction of yeast-like development in Mucor by carbon dioxide. Journal of Bacteriology 84:829–840
     [Google Scholar]
  3. Bartnicki-García S., Nickerson W. J. 1962b; Nutrition, growth and morphogenesis of Mucor rouxii. Journal of Bacteriology 84:841–858
     [Google Scholar]
  4. Bonnichsen R. 1963; Ethanol determination with alcohol dehydrogenase and DPN. In Methods of Enzymatic Analysis pp 285–287 Bergmeyer H. -U. Edited by New York & London: Academic Press;
     [Google Scholar]
  5. Carbonell L. M., Rodríguez J. 1965; Transformation of mycelial and yeast forms of Paracoccidioides brasiliensis in cultures and in experimental inoculations. Journal of Bacteriology 90:504–510
     [Google Scholar]
  6. Crocken B., Tatum E. L. 1968; The effect of sorbose on metabolism and morphology of Neurospora. Biochimica et biophysica acta 156:1–8
     [Google Scholar]
  7. Friedenthal M., Roselino E., Passeron S. 1973; Multiple molecular forms of pyruvate kinase from Mucor rouxii. Immunological relationship among the three isoenzymes and nutritional factors affecting the enzymatic pattern. European Journal of Biochemistry 35:148–158
     [Google Scholar]
  8. Haidle C. W., Storck R. 1966; Control of dimorphism in Mucor rouxii. Journal of Bacteriology 92:1236–1244
     [Google Scholar]
  9. Lowry O. H., Rosebrough A. L., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
     [Google Scholar]
  10. Lynen F., Hartmann G., Netter K. F., Schuegraff A. 1959; Phosphate turnover and the Pasteur effect. In Ciba Foundation Symposium on the Regulation of Cell Metabolism pp 256–276 Wolstenholme G. E. W., O’Connor C. M. Edited by London: Churchill;
     [Google Scholar]
  11. Smith L. 1955; Spectrophotometric assay of cytochrome c oxidase. In Methods of Biochemical Analysis 2 pp 427–434 Glick D. Edited by New York: Interscience Publishers;
     [Google Scholar]
  12. Terenzi H. F., Storck R. 1969; Stimulation of fermentation and yeast-like morphogenesis in Mucor rouxii by phenethyl alcohol. Journal of Bacteriology 97:1248–1261
     [Google Scholar]
  13. Wainio W. W., Cooperstein S. J. 1956; Some controversial aspects of the mammalian cytochromes. In Advances in Enzymology 17 pp 329–392 Nord F. F. Edited by New York: Interscience Publishers;
     [Google Scholar]
/content/journal/micro/10.1099/00221287-82-1-15
Loading
Effect of Potassium Cyanide, Glucose and Anaerobiosis on Morphogenesis of Mucor rouxii
Microbiology 82, 15 (1974); https://doi.org/10.1099/00221287-82-1-15
/content/journal/micro/10.1099/00221287-82-1-15
/content/journal/micro/10.1099/00221287-82-1-15
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error