The growth rate of in laboratory media could be stimulated or inhibited by the addition of appropriate concentrations of widely different potential nutrients and salts. The effect appeared to depend on the osmotic activity of the supplements since, with electrolytes, growth was maximal at about the same osmolal concentration irrespective of the chemical nature of the electrolyte. With sucrose a higher osmolality was required. In comparative tests the growth rate of was much less affected by variation in solute concentration. appears unusually sensitive to change in the osmolality of the growth medium and this may account for the general difficulty of obtaining satisfactory growth of the organism.


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