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Volume 80, Issue 1

Plasmids Formed in Nitrogen-fixing Hybrids Free

Abstract

SUMMARY

Segments of the chromosome, which included the nitrogen fixation () genes, were maintained as covalently closed circular molecules of DNA in hybrids, C-9 and c-4. The R factor, R 1443, which had been used to confer fertility on the donor, was also detected as covalently closed circular DNA of molecular weight (65 ± 4) × 10 daltons according to sedimentation studies and electron microscopy.

The genes in hybrid C-9 were linked to on a plasmid which had a molecular weight of 9·5 × 10 daltons. A third plasmid in this hybrid had a molecular weight of ii8 × 10 daltons. The Nif plasmid in C-9 was transferred efficiently to other strains only when R 119 was present in the donor in addition to R 1443.

Presence of three electrophoretically distinguishable gluconate-6-phosphate dehydrogenases was evidence for duplication of determinants in C-9. Resistance of both hybrids to the rough-specific phage X 174 suggested that they inherited Klebsiella -linked genes.

Four plasmids were isolated from hybrid c-14, one of which was R 1443. Two plasmids, one carrying and and another G, were mobilized by R 1443. The molecular weights of the c-4 plasmids, excluding R 1443, were (105 ± 1) × 10, 46 × 10 and 12 × 10 daltons.

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© Society for General Microbiology, 1974
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1974-01-01
2024-04-26
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Plasmids Formed in Nitrogen-fixing Escherichia coli-Klebsiella pneumoniae Hybrids
Microbiology 80, 241 (1974); https://doi.org/10.1099/00221287-80-1-241
/content/journal/micro/10.1099/00221287-80-1-241
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