1887

Abstract

SUMMARY

Segments of the chromosome, which included the nitrogen fixation () genes, were maintained as covalently closed circular molecules of DNA in hybrids, C-9 and c-4. The R factor, R 1443, which had been used to confer fertility on the donor, was also detected as covalently closed circular DNA of molecular weight (65 ± 4) × 10 daltons according to sedimentation studies and electron microscopy.

The genes in hybrid C-9 were linked to on a plasmid which had a molecular weight of 9·5 × 10 daltons. A third plasmid in this hybrid had a molecular weight of ii8 × 10 daltons. The Nif plasmid in C-9 was transferred efficiently to other strains only when R 119 was present in the donor in addition to R 1443.

Presence of three electrophoretically distinguishable gluconate-6-phosphate dehydrogenases was evidence for duplication of determinants in C-9. Resistance of both hybrids to the rough-specific phage X 174 suggested that they inherited Klebsiella -linked genes.

Four plasmids were isolated from hybrid c-14, one of which was R 1443. Two plasmids, one carrying and and another G, were mobilized by R 1443. The molecular weights of the c-4 plasmids, excluding R 1443, were (105 ± 1) × 10, 46 × 10 and 12 × 10 daltons.

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/content/journal/micro/10.1099/00221287-80-1-241
1974-01-01
2022-01-23
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References

  1. Baron L. S., Gemski P.Jun Johnson E. M., Wohlhieter J. A. 1968; Intergeneric bacterial matings. Bacteriological Reviews 32:362–369
    [Google Scholar]
  2. Bazaral M., Helinski D. R. 1968; Characterization of multiple circular DNA forms of colicinogenic factor El from Proteus mirabilis. Biochemistry 7:3513–3519
    [Google Scholar]
  3. Cannon F. C., Dixon R. A., Postgate J. R., Primrose S. B. 1974; Chromosomal integration of Klebsiella nitrogen fixation genes in Escherichia coli. Journal of General Microbiology 80:227–239
    [Google Scholar]
  4. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplexmethods for mapping regions of base sequency homology in nucleic acids. In Methods in Enzymology 21 pp. 413–428 Grossman L., Moldave K. Edited by New York and London: Academic Press;
    [Google Scholar]
  5. Dixon R. A., Postgate J. R. 1971; Transfer of nitrogen fixation genes by conjugation in Klebsiella pneumoniae. Nature; London: 23447–48
    [Google Scholar]
  6. Dixon R. A., Postgate J. R. 1972; Genetic transfer of nitrogen fixation from Klebsiella pneumoniae to Escherichia coli. Nature; London: 237102–103
    [Google Scholar]
  7. Falkow S., Schneider H., Baron L. S., Formal S. B. 1963; Virulence of Escherichia-Shigella genetic hybrids for the guinea pig. Journal of Bacteriology 86:1251–1258
    [Google Scholar]
  8. Gemski P.Jun Wohlheiter J. A., Baron L. S. 1967; Chromosome transfer between Escherichia coli Hfr strains and Proteus mirabilis. Proceedings of the National Academy of Sciences of the United States of America 58:1461–1467
    [Google Scholar]
  9. Leavitt R. W., Wohlieter J. A., Johnson E. M., Alson G. E., Baron L. S. 1971; Isolation of circular deoxyribonucleic acid from Salmonella typhosa hybrids obtained from matings with Escherichia coli Hfr donors. Journal of Bacteriology 108:1357–1365
    [Google Scholar]
  10. Mojica-a T., Middleton R. B. 1972; Salmonella typhimurium-Escherichia coli hybrids for the tryptophan region. Genetics 71:491–505
    [Google Scholar]
  11. Postgate J. R. 1971; Fixation by free-living microbes: physiology. In The Chemistry and Biochemistry of Nitrogen Fixation pp. 161–190 Postgate J. R. Edited by London: Plenum Press;
    [Google Scholar]
  12. Radke K. L., Siegel E. C. 1971; Mutation preventing capsular polysaccharide synthesis in Escherichia coli K12 and its effects on bacteriophage resistance. Journal of Bacteriology 106:432–437
    [Google Scholar]
  13. Streicher S. L., Gurney E. G., Valentine R. C. 1971; Transduction of the nitrogen fixation genes in Klebsiella pneumonia. Proceedings of the National Academy of Sciences of the United States of America 68:1174–1177
    [Google Scholar]
  14. Taylor A. K., Trotter C. D. 1972; Linkage map of Escherichia coli strain K12. Bacteriological Reviews 36:504–524
    [Google Scholar]
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