SUMMARY: HS was evolved from soil treated with CCl when moistened with sucrose and (NH)SO solution and incubated aerobically. HS formation took place when the soil moisture was less than field capacity, and over a range of pH values from 5 to 8. The organism responsible was isolated and identified as Several strains of this organism reduced sulphate in well-aerated sterilized soil and liquid media but not in soil or liquid incubated anaerobically.

The action of CCl in fresh soil is to check or destroy certain fungi and bacteria which normally inhibit sulphate reduction by Some of these organisms were isolated and shown to be sensitive to CCl and to inhibit sulphate reduction by in sterilized soil. The isolates did not exhibit antibiotic action when grown in certain defined media.


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