1887

Abstract

SUMMARY: From an strain 12 carrying a temperature-sensitive hostspecific modification () mutation, second-step mutants have been isolated that are completely deficient in modification and restriction. Complementation analysis has revealed that one group of these mutants is impaired in the specificity gene , while in the other group of mutant strains both mutations, i.e. the first-step temperature-sensitive and the second-step which impairs restriction and modification completely, are located in . Analysis of the heterozygotes used in the complementation experiments suggested a - and tandem arrangement of the and genes in haploid, segregating exconjugants; however, the attachment of these genes to a cryptic plasmid was not excluded.

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/content/journal/micro/10.1099/00221287-79-2-257
1973-12-01
2021-08-04
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