1887

Abstract

Summary: Mutants, designated deficient in NADP-glutamate dehydrogenase activity grow poorly using inorganic nitrogen but normally using amino acids as nitrogen sources. The mutants are not repressed by ammonia for a number of enzyme and uptake systems. The nine mutations are allelic and located in linkage group III; is recessive in the heterozygous diploid with respect to both NADP glutamate dehydrogenase activity and ammonium regulation.

Mutants, designated deficient in NAD-glutamate dehydrogenase activity are unable to utilize -glutamate as a carbon source, but can utilize inorganic and organic nitrogen as nitrogen sources. The four mutations are allelic, located in linkage group IV, and is recessive in the heterozygous diploid. The mutants are repressed for the ammonium regulated systems which are not repressed in mutants.

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/content/journal/micro/10.1099/00221287-78-1-39
1973-09-01
2024-04-26
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References

  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimum conditions for mutagenesis by >N>-methyl- >N>′-nitro->N>-nitrosoguanidine in Escherichia coli k12.. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Arst H. N., Cove D. J. 1969; Methylammonium resistance in Pseudomonas aeruginosa.. Journal of Bacteriology 98:1284–1293
    [Google Scholar]
  3. Cohen B. L. 1972; Ammonium repression of extracellular protease in Pseudomonas aeruginosa.. Journal of General Microbiology 71:293–299
    [Google Scholar]
  4. Cove D. J. 1966; The induction and repression of nitrate reductase in the fungus Pseudomonas aeruginosa.. Biochimica et biophysica acta 113:51–56
    [Google Scholar]
  5. Dunn E., Pateman J. A. 1972; Urea and thiourea uptake in Pseudomonas aeruginosa.. Heredity 29:129
    [Google Scholar]
  6. Fincham J. R. S. 1962; Genetically determined multiple forms of glutamic dehydrogenase in Pseudomonas aeruginosa.. Journal of Molecular Biology 4:257
    [Google Scholar]
  7. Grenson M., Hou C. 1972; Ammonia inhibition of the general amino acid permease and its suppression in NADP-specific glutamate dehydrogenase mutants of Pseudomonas aeruginosa.. Biochemical and Biophysical Research Communications 48:749–756
    [Google Scholar]
  8. Herman C., Clutterbuck A. J. 1966; A method for selection of auxotrophs by means of ‘spidery’ growth.. Aspergillus Newsletter 7:13–14
    [Google Scholar]
  9. Hynes M. J., Pateman J. A. 1970a; The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. I. Mutants unable to utilise acrylamide.. Molecular and General Genetics 108:97–106
    [Google Scholar]
  10. Hynes M. J., Pateman J. A. 1970b; The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. II. Mutants resistant to fluoroacetamide.. Molecular and General Genetics 108:107–116
    [Google Scholar]
  11. Kinghorn J. R., Pateman J. A. 1972; Regulation of glutamate transport in Pseudomonas aeruginosa.. Heredity 29:128
    [Google Scholar]
  12. LéJohn H. B. 1971; Enzyme regulation, lysine pathways and cell wall structures as indicators of major lines of evolution in fungi.. Nature, London 231:164–168
    [Google Scholar]
  13. Lowry O. H., Rosebrough M. J., Farr J. C., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Mackintosh M. E., Pritchard R. H. 1963; The production and replica plating of micro-colonies of Pseudomonas aeruginosa.. Genetical Research, Cambridge 4:320–322
    [Google Scholar]
  15. McCully K. S., Forbes E. 1965; Use of p-fluorophenylalanine with master strains of Aspergillus nidulans.. Genetical Research, Cambridge 6:352–359
    [Google Scholar]
  16. Pateman J. A. 1969; Regulation of the synthesis of glutamic dehydrogenase and glutamine synthetase in micro-organisms.. Biochemical Journal 115:769
    [Google Scholar]
  17. Pateman J. A., Cove D. J. 1967; Regulation of nitrate reduction in Pseudomonas aeruginosa.. Nature, London 215:1234–1239
    [Google Scholar]
  18. Pontecorvo G., Roper J. A., Hemmons L. M., MacDonald K. D., Bufton A. W. J. 1953; The genetics of Pseudomonas aeruginosa.. Advances in Genetics 5:141–238
    [Google Scholar]
  19. Scazzocchio C., Darlington A. J. 1968; The induction and repression of the enzymes of purine breakdown in Pseudomonas aeruginosa.. Biochimica et biophysica acta 166:557–568
    [Google Scholar]
  20. Wootton J. C., Chambers G. K., Taylor J. G., Fincham J. R. S. 1973; Amino-acid sequence homologies between the NADP-dependent glutamate dehydrogenase of Neurospora and the bovine enzyme Nature New Biology . 241:42–43
    [Google Scholar]
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