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SUMMARY: Data from two experiments indicated that transformation occurred in Candida pseudotropicalis in the conversion of lactose-negative to lactose-positive cells. When the DNA from prototrophic cells was mixed in media with auxotrophic cells approximately 0.1% of the recipient cells were transformed to prototrophs.
Transformation activity of the donor DNA was found to diminish with heat, DNase or 2-deoxyglucose. The frequencies of transformation obtained in this study compare with those observed in other fungi and some bacteria.
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