1887

Abstract

SUMMARY: Growing cultures of 1477 hydroxylate progesterone at the 11-, 17- and 6-positions. Hydroxylation at the 11-position seems to be predominant. At pH 7.5, extracts of this organism catalysed the hydroxylation of progesterone to 11-hydroxyprogesterone and 11-hydroxyallopregnane-3,20-dione. Ethylenediamine tetracetate inhibited and 2-mercaptoethanol stimulated this hydroxylation. While hydroxylation of progesterone at the 11-position was optimal at pH 7.5, hydroxylation at the 17-position was optimal at pH 6.5. The effect of some compounds and metal salts was studied.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-77-2-465
1973-08-01
2024-11-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/77/2/mic-77-2-465.html?itemId=/content/journal/micro/10.1099/00221287-77-2-465&mimeType=html&fmt=ahah

References

  1. Caper A., Tadra M., Tuma J. 1964; Separation of androst-17-hydroxy-epimers. Folia microbiologica 9380–382
    [Google Scholar]
  2. Chang F. N., Sih C. J. 1964; Mechanisms of steroid oxidation by microorganisms. VII. Properties of the 9<x-hydroxylase. Biochemistry 3:1551–1557
    [Google Scholar]
  3. Hayaishi O. 1969; Enzymic hydroxylation. Annual Review of Biochemistry 38:21–44
    [Google Scholar]
  4. Nguyen-Dang-Tam Mayer M., Janot M. M. 1971; Hydroxylation of progesterone by a cell-free extract of Pseudomonas aeruginosa. Compte rendu des séances de VAcademie des sciences D 272:2032–2033
    [Google Scholar]
  5. Sallam L. A. R., , El-Refai A. H., El-Kady I. A. 1971; The in vitro transformation of progesterone by Rhizopus nigricans REF 129. Zeitschrift für allgemeine Mikrobiologie 11:325–330
    [Google Scholar]
  6. Wilson J. A., Vestling C. S. 1965; A cell-free steroid hydroxylating system from Bacillus megaterium, strain KM. Archives of Biochemistry and Biophysics no, 401–403
    [Google Scholar]
  7. Zuidweg M. H. J., , Van Der Waard W. F., DeFlines J. 1962; Formation of hydrocortisone by hydroxylation of Reichstein’s compound S. with an enzyme preparation from Pseudomonas aeruginosa. Biochimica et biophysica acta 58:131–133
    [Google Scholar]
  8. Zuidweg M. H. J. 1968; Hydroxylation of Reichstein’s compound S with cell-free preparation from Pseudomonas aeruginosa. Biochimica et biophysica acta 152:144
    [Google Scholar]
/content/journal/micro/10.1099/00221287-77-2-465
Loading
/content/journal/micro/10.1099/00221287-77-2-465
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error