1887

Abstract

SUMMARY: Growing cultures of 1477 hydroxylate progesterone at the 11-, 17- and 6-positions. Hydroxylation at the 11-position seems to be predominant. At pH 7.5, extracts of this organism catalysed the hydroxylation of progesterone to 11-hydroxyprogesterone and 11-hydroxyallopregnane-3,20-dione. Ethylenediamine tetracetate inhibited and 2-mercaptoethanol stimulated this hydroxylation. While hydroxylation of progesterone at the 11-position was optimal at pH 7.5, hydroxylation at the 17-position was optimal at pH 6.5. The effect of some compounds and metal salts was studied.

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/content/journal/micro/10.1099/00221287-77-2-465
1973-08-01
2021-08-01
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