1887

Abstract

SUMMARY: Liver and spleen tissue from mice infected 4 to 5 months previously with has been examined in the electron microscope by negative staining, freeze-etching and ultrathin sectioning. Part or all of the electron-transparent zone seen around sectioned bacteria is composed of parallel fibrils wrapped longitudinally around the bacteria. It was isolated from homogenates of infected livers and spleens using urea density gradients and contained a mycoside of type C, a peptidoglycolipid known to occur in some other species of mycobacteria. The mycoside therefore forms a capsule around the bacterium to protect and insulate it from the host cell and its lysosomes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-77-1-79
1973-07-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/77/1/mic-77-1-79.html?itemId=/content/journal/micro/10.1099/00221287-77-1-79&mimeType=html&fmt=ahah

References

  1. Allen J. M., Brieger E. M., Rees R. J. W. 1965; Electron microscopy of the host-cell parasite relation in murine leprosy. Journal of Pathology and Bacteriology 89:301–306
    [Google Scholar]
  2. Armstrong J. A., Hart P. D’Arcy. 1971; Response of cultured macrophages to Mycobacterium tuberculosis, with observations on fusion of lysosomes with phagosomes. Journal of Experimental Medicine 134:713–740
    [Google Scholar]
  3. Brown C. A., Draper P. 1970; An electron-microscope study of rat fibroblasts infected with Pseudomonas aeruginosa. Journal of Pathology 102:21–26
    [Google Scholar]
  4. Chapman G. B., Hanks J. H., Wallace J. H. 1959; An electron microscope study of the disposition and fine structure of Mycobacterium lepraemurium in mouse spleen. Journal of Bacteriology 77:205–211
    [Google Scholar]
  5. Chaput M., Michel G., Lederer E. 1963; Structure du mycoside C2 de Mycobacterium avium. Biochimica et biophysica acta 78:329–341
    [Google Scholar]
  6. Dische Z. 1962; Colour reactions of 6-deoxy, 3-deoxy and 3,6-dideoxyhexoses. In Methods in Carbohydrate Chemistry vol 1 pp 501–503 Edited by Whistler R. L., Wolfrom M. L. New York: Academic Press;
    [Google Scholar]
  7. Draper P. 1971; The walls of Mycobacterium lepraemurium: chemistry and ultrastructure. Journal of General Microbiology 69:313–324
    [Google Scholar]
  8. Draper P., Rees R. J. W. 1970; Electron-transparent zone of mycobacteria may be a defence mecha. Nature, London 228:860–861
    [Google Scholar]
  9. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  10. Evans M. J., Levy L. 1972; Ultrastructural changes in cells of the mouse footpad infected with Pseudomonas aeruginosa. Infection and Immunity 5:238–247
    [Google Scholar]
  11. Furuchi A., Tokunaga T. 1972; Nature of the receptor substance of Mycobacterium smegmatis for D4 bacteriophage adsorption. Journal of Bacteriology in 404–411
    [Google Scholar]
  12. Goren M. B. 1972; Mycobacterial lipids: selected topics. Bacterial Reviews 36:33–64
    [Google Scholar]
  13. Goren M. B., McClatchy J. K., Martens B., Brokl O. 1972; Mycosides C: behaviour as receptor site substance for mycobacteriophage D4. Journal of Virology 9:999–1003
    [Google Scholar]
  14. Hanks J. H. 1961; The origin of the capsules on Mycobacterium leprae and other tissue-grown mycobacteria. International Journal of Leprosy 29:172–174
    [Google Scholar]
  15. Hasegawa T. 1971; Further electron-microscopic observations of Pseudomonas aeruginosa. Dermatologica 143:363–367
    [Google Scholar]
  16. Kellenberger E., Ryter A., Sechaud J. 1958; Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. Journal of Biophysical and Biochemical Cytology 4:671–678
    [Google Scholar]
  17. Lederer E. 1967; Glycolipids of mycobacteria and related microorganisms. Chemistry and Physics of Lipids 1:294–315
    [Google Scholar]
  18. Merckx J. J., Brown A. L. Jun, Karlson A. G. 1964; An electron-microscopic study of experimental infections with acid-fast bacilli. American Review of Respiratory Diseases 89:485–496
    [Google Scholar]
  19. Nishiura M. 1960; The electron microscopic basis of the pathology of leprosy. International Journal of Leprosy 28:357–400
    [Google Scholar]
  20. Rees R. J. W., Valentine R. C., Wong P. C. 1960; Application of quantitative electron microscopy to the study of Mycobacterium lepraemurium and M. leprae. Journal of General Microbiology 22:443–457
    [Google Scholar]
  21. Smith D. W., Randall H. H., MacLennan A. P., Putney R. K., Rao S. V. 1960; Detection of specific lipids in mycobacteria by infrared spectroscopy. Journal of Bacteriology 79:217–229
    [Google Scholar]
  22. Takeya K., Hisatsune K. 1963; Mycobacterial cell walls. I. Methods of preparation and treatment with various chemicals. Journal of Bacteriology 85:16–23
    [Google Scholar]
  23. Vilkas E., Rojas A., Das B. C., Wolstenholme W. A., Lederer E. 1966; Détermination de séquences d’acidés amines dans des oligopeptides par la spectrométrie de masse. VI. Structures du mycoside C2, peptidoglycolipide de Mycobacterium butyricum. Tetrahedron 22:2809–2821
    [Google Scholar]
  24. Voiland A., Bruneteau M., Michel G. 1971; Étude du mycoside C2 de Mycobacterium avium. European Journal of Biochemistry 21:285–291
    [Google Scholar]
  25. Walker R. W., Mallmann W. L., Brunner J. R. 1967; Type-specific lipids of mycobacteria. American Review of Respiratory Diseases 95:1065–1067
    [Google Scholar]
  26. Whitehouse R. L. S., Wong P. C., Jackson F. L. 1971; Ultrastructure of Pseudomonas aeruginosa. International Journal of Leprosy 39:151–163
    [Google Scholar]
  27. Yamamoto T., Nishiura M., Harada N., Imaeda T. 1958a; Electron microscopy of Mycobacterium lepraemurium in ultrathin sections of murine leprosy lesions. International Journal of Leprosy 26:111–114
    [Google Scholar]
  28. Yamamoto T., Nishiura M., Harada N., Imaeda T. 1958b; Electron microscopy of ultra-thin sections of lepra cells and Mycobacterium leprae. International Journal of Leprosy 26:1–8
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-77-1-79
Loading
/content/journal/micro/10.1099/00221287-77-1-79
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error