1887

Abstract

Summary: bacteria have a higher spontaneous mutability and are 4.6 times more u.v.-sensitive than isogenic wild-type bacteria. Bacteria deficient in dimer excision () are 15 times more u.v.-sensitive than isogenic bacteria. Double mutant bacteria are only 1.5 times more u.v.-sensitive than bacteria and double mutant bacteria were as u.v.-sensitive as bacteria, suggesting that there exists a - and - dependent repair pathway which is blocked by the mutation. We suggest that the mutation affects primarily the excision repair pathway. Strict additivity of the and mutations on u.v. sensitivity suggests that -mediated repair acts in part of repair pathway different from excision repair. U.v.-induced DNA degradation in the bacteria is only slightly greater than that in bacteria and the mutation does not affect the ဘrecklessမ character of u.v.-induced DNA breakdown in the bacteria. The and or double mutants have spontaneous mutation frequencies similar to that of the single strain.

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/content/journal/micro/10.1099/00221287-76-2-407
1973-06-01
2021-07-26
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References

  1. Böhme H. 1969; In discussion in Mutation as a Cellular Process . pp 206–209 Ciba Foundation Symposium Edited by Wolstenholme G. E. W., O’Connor M. London: J. and A. Churchill;
    [Google Scholar]
  2. Boyce R. P., Howard-Flanders P. 1964; Release of ultraviolet light-induced thymine dimers from DNA in E. coli k-12. Proceedings of the National Academy of Sciences of the United States of America 51:293–300
    [Google Scholar]
  3. Bridges B. A. 1972; Evidence for a further dark repair process in bacteria. Nature New Biology 240:52–53
    [Google Scholar]
  4. Cooper P. K., Hanawalt P. C. 1972; Role of DNA polymerase I and the Rec system in excision repair in Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 65:1156–1160
    [Google Scholar]
  5. Davis B., Mingioli E. 1950; Mutants of Escherichia coli requiring methionine or vitamin B 12. Journal of Bacteriology 60:17–28
    [Google Scholar]
  6. Demerec M. E., Adelberg E. A., Clark A. J., Hartman P. E. 1966; A proposal for a uniform nomenclature in bacterial genetics. Genetics 54:61–76
    [Google Scholar]
  7. Drake J. W. 1969; Mutagenic mechanisms. Annual Review of Genetics 3:247–268
    [Google Scholar]
  8. Gross J. D., Grunstein J., Witkin E. M. 1971; Inviability of recA derivatives of the DNA polymerase mutant of de Lucia and Cairns. Journal of Molecular Biology 58:631–634
    [Google Scholar]
  9. Horii Z.-I., Suzuki K. 1968; Degradation of the DNA of E. coli k-12 rec (JC1579b) after irradiation with ultraviolet light. Photochemistry and Photobiology 8:93–105
    [Google Scholar]
  10. Howard-Flanders P., Boyce R. P. 1966; DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiation Research Supplement 6:156–184
    [Google Scholar]
  11. Kato T., Kondo S. 1970; Genetic and molecular characteristics of X-ray-sensitive mutants of Escherichia coli defective in repair synthesis. Journal of Bacteriology 104:871–881
    [Google Scholar]
  12. Klein A., Niebch U. 1971; Host cell reactivation in strains of E. coli lacking DNA polymerase activity in vitro . Nature New Biology 229:82–84
    [Google Scholar]
  13. Liberfarb R. M., Bryson V. 1970; Isolation, characterization and genetic analysis of mutator genes in Escherichia coli b and k-12. Journal of Bacteriology 104:363–375
    [Google Scholar]
  14. Mattern I. E. 1971; A class of UV-sensitive mutants of E. coli k-12 that show a high spontaneous mutation frequency. In First European Biophysics Congress pp 237–240 Edited by Broda E., Locker A., Springer-Lederer H.
    [Google Scholar]
  15. Monk M., Peacey M., Gross J. D. 1971; Repair of damage induced by ultraviolet light in DNA polymerase-defective Escherichia coli cells. Journal of Molecular Biology 58:623–530
    [Google Scholar]
  16. Paterson M. C., Boyle J. M., Setlow R. B. 1971; Ultraviolet and X-ray-induced responses of a deoxyribonucleic acid polymerase-deficient mutant of Escherichia coli . Journal of Bacteriology 107:61–67
    [Google Scholar]
  17. Siegel E. C. 1973; Ultraviolet-sensitive mutator of Escherichia coli k-12. Journal of Bacteriology 113:145–160
    [Google Scholar]
  18. Smirnov G. B., Filkova E. V., Ska Vronskaya A. G. 1972; The mutator property of uvr502 mutation affecting UV-sensitivity of Escherichia coli . Molecular and General Genetics 118:51–56
    [Google Scholar]
  19. Smirnov G. B., Filkova E. V., Skavronskaya A. G., Saenko A. S., Sinzinis B. I. 1973; Loss and restoration of viability of E. coli due to combinations of mutations affecting DNA polymerase I and repair activities. Molecular and General Genetics 121:139–150
    [Google Scholar]
  20. Smirnov G. B., Skavronskaya A. G. 1968; The lethal and mutagenic action of UV-light and mitomycin C on the mutants of Escherichia coli with reduced dark repair ability. Genetika (USSR) 4:105–110
    [Google Scholar]
  21. Smirnov G. B., Skavronskaya A. G. 1971; Location of uvr502 mutation on the chromosome of Escherichia coli k-12. Molecular and General Genetics 113:217–221
    [Google Scholar]
  22. Smith K. C., Meun D. H. C. 1970; Repair of radiation-induced damage in Escherichia coli. I. Effect of rec mutations on postreplication repair of damage due to ultraviolet radiation. Journal of Molecular Biology 51:459–472
    [Google Scholar]
  23. Taylor A. L. 1970; Current linkage map of Escherichia coli . Bacteriological Reviews 34:155–175
    [Google Scholar]
  24. Willetts N. S., Clark A. J. 1969; Characteristics of some multiply recombination-deficient strains of Escherichia coli . Journal of Bacteriology 100:231–239
    [Google Scholar]
  25. Yonei S., Nozu K. 1972; Mechanism of postirradiation degradation of deoxyribonucleic acid in a radiosensitive Escherichia coli (NG 30) irradiated with ultraviolet light. Journal of Molecular Biology 65:213–225
    [Google Scholar]
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