1887

Abstract

SUMMARY: The mycelial and blastospore forms of have been grown under conditions in which the only environmental variable was temperature. The activity of phosphoglucose isomerase, phosphofructokinase and the first enzyme of the hexose-monophosphate pathway and the pathways for chitin and mannan synthesis has been determined in extracts at different times in the cell cycle. Phosphofructokinase has been partially purified from both forms and the effect of adenosine phosphates on its activity determined. In this yeast, concentrations of glucose-6-phosphate, fructose-6-phosphate, ATP, ADP and AMP differed in the two growth forms and at different times in the growth cycle. L-Glutamine-D-fructose-6-phosphate aminotransferase activity at concentrations of fructose-6-phosphate found in the cell was appreciably greater in mycelium than in blastospores. The metabolism of [C]glucose through the hexose monophosphate pathway was low after 4 and 18 h growth, and considerably increased at 10 h. The results support the concepts of a requirement for NADPH for cell division and suggest that control over the synthesis of chitin and mannan may, in part, be provided through control of the activity of phosphofructokinase by adenosine phosphates.

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/content/journal/micro/10.1099/00221287-75-1-97
1973-03-01
2022-09-25
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