SUMMARY: 107 constitutively oxidizes -nonane and longer alkanes. Decane and longer alkanes have little effect on endogenous respiration (measured as CO expiration from yeast grown on [U-C]glucose). Alkane-grown yeast assimilated [1-C]decane more rapidly than glucose-grown yeast though without a lag in both cases. Glucose did not inhibit these incorporations. [U-C]Glucose was assimilated without a lag into glucose-grown yeast and, at a quarter of this rate, into alkane-grown yeast: if the latter yeast was pre-incubated with glucose for 60 min, it took up [U-C]glucose at the higher rate. -Decane (0.5 to 1.0 mg/mg yeast dry wt) severely and rapidly inhibited glucose assimilation. Longer chain alkanes were progressively less effective. All alkanes were more inhibitory with alkane-grown yeast. The rate of glucose assimilation was inversely proportional to the concentration of decane and directly proportional to the yeast concentration. Transport of glucose, as distinct from its assimilation, was also inhibited by decane. Uptake of fucose, a non-metabolizable deoxyhexose, was similarly affected. Passive transport of glucose did not occur. Alkanes did not prevent glucose access to the yeast surface as pristane, a non-metabolizable paraffin, had no effect on glucose assimilation in 107 and dodecane was not inhibitory against carbohydrate transport in Alkanes probably inhibit by causing accumulation of fatty acids or acyl CoA esters which may, either through feedback inhibitions or by further metabolism, cause build-up of ATP, acetyl CoA, and glucose 6-phosphate, thus leading to cessation of glucose transport and metabolism.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error