1887

Abstract

SUMMARY: Chemotaxis of a bacterium such as is assayed by measuring the number of organisms attracted into a capillary tube containing an attractant. Rate of bacterial accumulation in capillaries and a concentration-response curve for -aspartate taxis are presented and interpreted, and the effect of bacterial concentration is reported. Other parameters of the assay were studied, such as the volume of fluid in the capillary and the size of the capillary opening. The concentration gradient of chemical was also described. chemotaxis requires EDTA to allow motility, a buffer to maintain the pH at its optimum near neutrality, and -methionine if it cannot be synthesized. Under certain conditions there is stimulation by inorganic ions, either by K or, less effectively, by Na. Chemotaxis is dependent on temperature, there being a 20-fold increase in the number of bacteria accumulating in a capillary when the temperature is raised from 20 to 30 °C.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-74-1-77
1973-01-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/74/1/mic-74-1-77.html?itemId=/content/journal/micro/10.1099/00221287-74-1-77&mimeType=html&fmt=ahah

References

  1. Adler J. 1966; Chemotaxis in bacteria. Science, New York 153:708–716
    [Google Scholar]
  2. Adler J. 1969; Chemoreceptors in bacteria. Science, New York 166:1588–1597
    [Google Scholar]
  3. Adler J., Dahl M. M. 1967; A method for measuring the motility of bacteria and for comparing random and non-random motility. Journal of General Microbiology 46:161–173
    [Google Scholar]
  4. Adler J., Templeton B. 1967; The effect of environmental conditions on the motility of Escherichia coli. Journal of General Microbiology 46:175–184
    [Google Scholar]
  5. Berg H. C. 1971; How to track bacteria. Review of Scientific Instruments 42:868–871
    [Google Scholar]
  6. Berg H. C., Brown D. A. 1972; Chemotaxis in Escherichia coli: analysis by three-dimensional tracking. Nature, London (in the press)
    [Google Scholar]
  7. Dahlquist F. W., Lovely P., Koshland D. E. Jun. 1972; Quantitative analysis of bacterial migration in chemotaxis. Nature New Biology, London 236:120–123
    [Google Scholar]
  8. Dryl S. 1958; Photographic registration of movement of protozoa. Bulletin de l’Academic polonaise des sciences. Serie des Sciences biologiques 6:429–430
    [Google Scholar]
  9. Futrelle R. P., Berg H. C. 1972; Specification of gradients used for studies of chemotaxis. Nature, London in the press
    [Google Scholar]
  10. Hazelbauer G. L., Mesibov R. E., Adler J. 1969; Escherichia coli mutants defective in chemotaxi toward specific chemicals. Proceedings of the National Academy of Sciences of the United States of America 64:1300–1307
    [Google Scholar]
  11. Kaiser A. D., Hogness D. S. 1960; The transformation of Escherichia coli with deoxyribonucleic acid isolated from the bacteriophage Adg. Journal of Molecular Biology 2:392–415
    [Google Scholar]
  12. Koch A. L. 1964; The role of permease in transport. Biochimica et biophysica acta 79:177–200
    [Google Scholar]
  13. Mesibov R. 1970 Ph.D. Thesis University of Wisconsin; Madison, U.S.A.:
    [Google Scholar]
  14. Mesibov R., Ordal G. W., Adler J. 1973; The range of attractant concentration for bacterial chemotaxis and the threshold and size of response over this range: Weber law and related phenomena. Journal of General Physiology (in the press)
    [Google Scholar]
  15. Overath P., Schairer H. U., Stoffel W. 1970; Correlation of in vivo and in vitro phase transitions of membrane lipids in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 67:606–612
    [Google Scholar]
  16. Pfeffer W. 1884; Locomotorische Richtungsbewegungen durch Chemische Reize. Untersuchungen aus dem Botanischen Institut in Tubingen 1:363–482
    [Google Scholar]
  17. Pfeffer W. 1888; Über Chemotaktische Bewegungen von Bacterien, Flagellaten, und Volvocineen. Untersuchungen aus dem Botanischen Institut in Tubingen 2:582–661
    [Google Scholar]
  18. Vaituzis Z., Doetsch R. N. 1969; Motility tracks: technique for quantitative study of bacterial movement. Applied Microbiology 17:584–588
    [Google Scholar]
  19. Yokota T., Gots J. S. 1970; Requirement of adenosine-cyclic phosphate for flagella formation in Escherichia coli and Salmonella typhimurium. Journal of Bacteriology 103:513–516
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-74-1-77
Loading
/content/journal/micro/10.1099/00221287-74-1-77
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error