1887

Abstract

SUMMARY

A study was made of the incorporation of carbon from [C]methylamine by cultures of AMI growing on methylamine. The distribution of radioactivity within the non-volatile constituents of the ethanol-soluble fractions of the bacteria, after incubation for periods of up to 38 s, was analysed by chromato-graphy and autoradiography. Most of the radioactivity fixed at the earliest times of sampling appeared in serine, phosphorylated compounds and malate. These results contrasted with previous studies of the incorporation of labelled growth substrates by cultures of MS growing on methylamine or trimethyl-sulphonium salts and correlated with the specific activities of hydroxypyruvate reductase, phosphoepyruvate carboxylase, -methyl glutamate synthetase and γ-glutamylmethylamide synthetase in bacterial extracts. No hydroxypyruvate reductase was detectable in extracts of MS grown either on methylamine or on trimethylsulphonium nitrate. The assimilation pathway for methylamine in AMI appears essentially the same as that for methanol and different from that in MS.

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/content/journal/micro/10.1099/00221287-72-3-485
1972-10-01
2021-07-23
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References

  1. ANTHONY C., DUNSTAN P. M., DRABBLE W. T. 1971; Metabolism of Cx and C2 compounds by Pseudomonas AM I: a glycerate kinase mutant and a mutant defective in glyoxylate formation from Q compounds.. Biochemical Journal 125:66–67P
    [Google Scholar]
  2. BRAY G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter.. Analytical Biochemistry 1:279–285
    [Google Scholar]
  3. GROSS D. 1959; High-voltage paper electrophoresis of organic acids and determination of migration rates.. Chemistry and Industry 1219–1220
    [Google Scholar]
  4. HARDER W., QUAYLE J. R. 1971; Aspects of glycine and serine biosynthesis during growth of Pseudomonas AM I on Ci compounds.. Biochemical Journal 121:763–769
    [Google Scholar]
  5. HEPTINSTALL J., QUAYLE J. R. 1970; Pathways leading to and from serine during growth of Pseudomonas AM I on Cx compounds or succinate.. Biochemical Journal 117:563–572
    [Google Scholar]
  6. HORNIG D., WAGNER C. 1968; Utilization of one-carbon units: methylene H4-folate reductase from Pseudomonas MS.. Bacteriological Proceedings p. 115
    [Google Scholar]
  7. KUNG H. 1969 Utilization of one carbon unit: metabolism of methylanine by Pseudomonas MS.. Ph.D. Thesis, Vanderbilt University U.S.A.:
    [Google Scholar]
  8. KUNG H. F., WAGNER C. 1969; γ-Glutamylmethylamide. A new intermediate in the metabolism of methylamine.. Journal of Biological Chemistry 244:4136–4140
    [Google Scholar]
  9. KUNG H. F., WAGNER C. 1970; Oxidation of Q compounds by Pseudomonas sp. MS.. Biochemical Journal 116:357–365
    [Google Scholar]
  10. LARGE P. J., PEEL D., QUAYLE J. R. 1961; Microbial growth on Q compounds. 2. Synthesis of cell constituents by methanol-and formate-grown Pseudomonas AM I, and methanol-grown Hyphomicro-bium vulgare.. Biochemical Journal 81:470–480
    [Google Scholar]
  11. LARGE P. J., PEEL D., QUAYLE J. R. 1962; Microbial growth on Q compounds. 4. Carboxylation of phosphoenolpyruvate in methanol-grown Pseudomonas AM I .. Biochemical Journal 85:243–67P250
    [Google Scholar]
  12. LARGE P. J., QUAYLE J. R. 1963; Microbial growth on Q compounds. 5. Enzyme activities in extracts of Pseudomonas AM I.. Biochemical Journal 87:386–396
    [Google Scholar]
  13. LEADBETTER E. R., GOTTLIEB J. A. 1967; On methylamine assimilation in a bacterium.. Archiv fur Mikro-biologie 59:211–217
    [Google Scholar]
  14. MYERS W. F., HUANG K. 1969; In Methods in Enzymology. vol 13:pp. 431–434 Edited by Lowenstein J. M. New York and London: Academic Press.;
    [Google Scholar]
  15. SHAW W. V., STADTMAN E. R. 1970; In Methods in Enzymology. vol 12: pp. 868–873 Edited by Tabor H., Tabor C. W. New York and London: Academic Press.;
    [Google Scholar]
  16. SHAW W. V., TSAI L., STADTMAN E. R. 1966; The enzymatic synthesis of N-methylglutamic acid.. Journal of Biological Chemistry 241:935–945
    [Google Scholar]
  17. I Present address: Veterans Administration Hospital, Department of Microbiology, 1310 Twenty Fourth Avenue South, Nashville, Tennessee 37203, U.S A.:
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