1887

Abstract

SUMMARY

The inhibition by triazole of the growth of wild-type is reversed by serine, methionine or cysteine. Two auxotrophs which responded to any one of these three compounds were hypersensitive to triazole and were shown by genetical analysis and enzyme assays to have a (serine transacetylase) deficiency. Triazole hypersensitivity of the mutants was reversed by sulphite and sulphide and triazole prevented induction of sulphate permease and activating enzymes by -acetyl serine (OAS) or serine in the wild-type. It is probable that the inhibitory effect of triazole is due to this interference with the induction of cysteine biosynthetic enzymes.

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/content/journal/micro/10.1099/00221287-72-2-291
1972-09-01
2021-08-04
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References

  1. ANTON D. 1968; Histidine regulatory mutants of Salmonella typhimurium. V. Two new classes of histidine regulatory mutants.. Journal of Molecular Biology 33:533–546
    [Google Scholar]
  2. BOGUSLAWSKI J., WALCZAK W., KLOPOTOWSKI T. 1967; Reversal by serine and potentiation by glycine of the inhibitory effect of 1,2,4‐triazole on growth of Salmonella typhimurium.. Acta biochimica polonica 14:133–141
    [Google Scholar]
  3. DREYFUSS J., PARDEE A. B. 1966; Regulation of sulphate transport in Salmonella typhimurium.. Journal of Bacteriology 91:2275–2280
    [Google Scholar]
  4. HILTON J. L., KEARNEY P. C., AMES B. N. 1965; Mode of action of the herbicide, 3‐amino‐1,2,4‐triazole (Amitrole) ; inhibition of an enzyme of histidine biosynthesis.. Archives of Biochemistry and Biophysics 112:562–566
    [Google Scholar]
  5. HULANICKA D., KLOPOTOWSKI T., BAGDASARIAN G. 1969; Inhibition of aminoimidazole ribotide bio‐synthesis in Salmonella typhimurium by aminotriazole.. Acta biochimica polonica 16:127–133
    [Google Scholar]
  6. JONES‐MORTIMER M. C. 1968a; Positive control of sulphate reduction in Escherichia coli. Isolation, characterisation and mapping of cysteineless mutants of E. coli K12.. Biochemical Journal 110:579–595
    [Google Scholar]
  7. JONES-MORTIMER M. C. 1969b; Positive control of sulphate reduction in Escherichia coli. The nature of the pleiotropic cysteineless mutants of E. coli K12.. Biochemical Journal 110:597–602
    [Google Scholar]
  8. KREDICH N. M., BECKER M. A., TOMKINS G. M. 1969; Purification and characterisation of cysteine synthetase, a bifunctional protein complex, from Salmonella typhimurium.. Journal of Biological Chemistry 244:2428–2439
    [Google Scholar]
  9. KREDICH N. M., TOMKINS G. H. 1966; The enzymic synthesis of L‐cysteine in Escherichia coli and Salmonella typhimurium.. Journal of Biological Chemistry 241:4955–4965
    [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. PASTERNAK C. A. 1962; Sulphate activation and its control in Escherichia coli and Bacillus subtilis.. Bio‐chemical Journal 85:44–49
    [Google Scholar]
  12. QURESHI M. A. 1971; Genetical and biochemical aspects of the relationship between cysteine and methionine synthesis in Salmonella tymphimurium. Ph.D. Thesis, Birmingham University
    [Google Scholar]
  13. ROBERTS R. B., COWIE D. B., ABELSON P. H., BOLTON E. T., BRITTEN R. J. 1963 Studies of Bio‐synthesis in Escherichia coli Washington: Carnegie Institution of Washington Publication; 607
    [Google Scholar]
  14. SANDERSON K. M. 1970; Current linkage map of Salmonella typhimurium.. Bacteriological Reviews 34:176–193
    [Google Scholar]
  15. SMITH D. A. 1971; S‐amino acid metabolism and its regulation in Escherichia coli and Salmonella typhimurium.. Advances in Genetics 16:141–165
    [Google Scholar]
  16. SMITH H. O. 1968; Defective phage formation by lysogens of integration deficient phage P22 mutants.. Virology 34:203–223
    [Google Scholar]
  17. SPENCER H. T., COLLINS J., MONTY K. J. 1967; Sequential regulation of cysteine biosynthesis in Salmonella typhimurium.. Federation Proceedings 26:677
    [Google Scholar]
  18. VOGEL H. J., BONNER D. M. 1956; Acetylornithase of Escherichia coli: partial purification and some properties.. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  19. WIEBERS J. L., GARNER H. R. 1967; Acyl derivatives of homoserine as substrates for homocysteine synthesis in Neurospora crassa, yeast and Escherichia coli.. Journal of Biological Chemistry 242:5644–5649
    [Google Scholar]
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