1887

Abstract

SUMMARY: Highest levels of β-galactosidase specific activity found after protracted growth of (syn. ) in lactose-limited chemostat culture were only developed with cultures previously conditioned to lactose in batch culture. The hyperactivities were not induced further by methyl thiogalactoside (MTG) and depended on dilution rate, being maximal at = 0·25 h. They were associated with increased substrate transport and utilization and, apart from a transient disturbance, were not affected by addition of glucose, galactose or glucose-6-phosphate to the lactose-limiting medium. Maltose addition produced a greater transient effect than the substances above, while citrate, β±-methyl glucoside and 2-deoxy-glucose were inactive. Before hyperactivity developed, adding glucose led to a rapid decline and adding MTG led to a rapid increase in β-galactosidase activity. Methyl thiogalactoside exerted an inhibiting action at only the final stage, the bacterial yield being decreased, and its continued presence enabled glucose-adapted organisms to utilize glucose and lactose simultaneously. The lactose was utilized in two stages. β±-Galactosidase hyperactivity occurred after protracted growth in melibiose-limited conditions, but β-glucosidase hyperactivity did not develop in cellobiose-limited medium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-72-1-37
1972-08-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/72/1/mic-72-1-37.html?itemId=/content/journal/micro/10.1099/00221287-72-1-37&mimeType=html&fmt=ahah

References

  1. BAIDYA T. K. N., WEBB F. C., LILLY M. D. 1967; The utilization of mixed sugars in continuous fermentation. I.. Biotechnology and Bioengineering 9:195–204
    [Google Scholar]
  2. Burstein C., Cohn M., Kepes A., Monod J. 1965; Role du lactose et de ses produits metaboliques dans l´induction de Poperon lactose chez Escherichia coli.. Biochimica et biophysica acta 95:634–639
    [Google Scholar]
  3. Clark D. J., Marr A. G. 1964; Studies on the repression of β-galactosidase in Escherichia coli.. Biochimica et biophysica acta 92:85–98
    [Google Scholar]
  4. COHEN G. N., MONOD J. 1957; Bacterial permeases.. Bacteriological Reviews 21:169–194
    [Google Scholar]
  5. DEAN A. C. R. 1972 In Environmental Control of Cell Synthesis and Function. Edited by DEAN A. C. R., Pirt S. J., Tempest D. W. pp. 245259 London and New York: Academic Press;
    [Google Scholar]
  6. DEAN A. C. R., HINSHELWOOD C. 1966 Growth,. Function and Regulation in Bacterial Cells pp. 130–135 260-284 Oxford: Clarendon Press;
    [Google Scholar]
  7. DEAN A. C. R., RODGERS P. J. 1969; Steady state levels of dehydrogenases and α-and β-glucosidases in Klebsiella aerogenes.. Journal of General Microbiology 57:169–178
    [Google Scholar]
  8. DEAN A. C. R., ROGERS P. L. 1967; The cell size and macromolecular composition ofAerobacter aerogenes in various systems of continuous culture.. Biochimica et biophysica acta 148:267–279
    [Google Scholar]
  9. HARRISON D. E. F., PIRT S. J. 1965; Oxygen tension and glucose metabolism of Klebsiella aerogenes.. Journal of General Microbiology 41:ix–x
    [Google Scholar]
  10. HERZENBERG L. A. 1959; Studies on the induction of β-galactosidase in a cryptic strain of Escherichia coli.. Biochimica et biophysica acta 31:525–538
    [Google Scholar]
  11. HORIUCHI T., TOMIZAWA J., NOVICK A. 1962; Isolation and properties of bacteria capable of high rates of β-galactosidase synthesis.. Biochimica et biophysica acta 55:152–163
    [Google Scholar]
  12. Jacob F., Monod J. 1961; On the regulation of gene activity.. Cold Spring Harbor Symposia on Quantitative Biology 26:193–209
    [Google Scholar]
  13. Koch A. L. 1963; The inactivation of the transport mechanism for β-galactosides of Escherichia coli under various physiological conditions.. Annals of the New York Academy of Sciences 102:602–620
    [Google Scholar]
  14. Koch A. L. 1964; The role of permease in transport.. Biochimica et biophysica acta 79:177–200
    [Google Scholar]
  15. Lederberg J. 1950; The β-D-galactosidase of Escherichia coli strain K12.. Journal of Bacteriology 60:381–392
    [Google Scholar]
  16. LOOMIS W. F., MAGASANIK B. 1967; Glucose-lactose diauxie in Escherichia coli.. Journal of Bacteriology 93:1397–1401
    [Google Scholar]
  17. MANDELSTAM J. 1962; The repression of constitutive β-galactosidase in Escherichia coli by glucose and other carbon sources.. Biochemical Journal 82:489–493
    [Google Scholar]
  18. MONOD J., COHN M. 1952; La biosynthese induite des enzymes (adaptation enzymatique).. Advances in Enzymology 13:67–119
    [Google Scholar]
  19. MONOD J., PAPPENHEIMER A. M., COHEN-BAZIRE G. 1952; La cinetique de la biosynthese de la β-galactosidase chez E. coli consideree comme fonction de la croissance.. Biochimica et biophysica acta 9:648–660
    [Google Scholar]
  20. Novick A., Horiuchi T. 1961; Hyper production of β-galactosidase by Escherichia colibacteria.. Cold Spring Harbor Symposia on Quantitative Biology 26:239–245
    [Google Scholar]
  21. Pardee A. B., Prestidge L. S. 1961; The initial kinetics of enzyme induction.. Biochimica et biophysica acta 49:77–88
    [Google Scholar]
  22. RICHARDS N. 1969; Regulation of gratuitous β-galactosidase synthesis in Aerobacter aerogenesduring an adaptive process.. Journal of General Microbiology 55:361–370
    [Google Scholar]
  23. RICHARDS N., HINSHELWOOD C. 1961; Observations on the β-galactosidase activity of Bacterium lactis aerogenes (Aerobacter aerogenes).. Proceedings of the Royal Society B 154:463–477
    [Google Scholar]
  24. RICHARDS N., HINSHELWOOD C. 1962; Stabilization of β-gaIactosidase in Bacterium lactis aerogenes.. Proceedings of the Royal Society B 156:20–40
    [Google Scholar]
  25. SILVER R. S., MATELES R. I. 1969; Control of mixed-substrate utilization in continuous cultures of Escherichia coli.. Journal of Bacteriology 97:535–543
    [Google Scholar]
  26. STRANGE R. E. 1961; Induced enzyme synthesis in aqueous suspensions of stationary phase Aerobacter aerogenes.. Nature, London 191:1272–1274
    [Google Scholar]
  27. TEMPEST D. W., Dicks J. W. 1967 In Microbial Physiology and Continuous Culture,. Edited by Powell E. O., Evans C. G. T., Strange R. E., Tempest D. W. pp. 140153 London: Her Majesty's Stationery Office.;
    [Google Scholar]
  28. VOJHSEK V., SIKYTA B. C., SLEZAK J. 1969 Regulation of the hyper production of β-galactosidase in continuous culture of Escherichia coli B.. In Continuous Cultivation of Micro-organisms Edited by Malek I., Beran K., Fencl Z., Munk V., Ricica J., Smrckova H. pp. 215223 Prague Academia London: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-72-1-37
Loading
/content/journal/micro/10.1099/00221287-72-1-37
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error