1887

Abstract

SUMMARY: The morphology of normal and 5-bromouracil (5-BU) containing strain was examined after exposure of the bacteria to X-rays (under aerobic and anoxic conditions) and to ultraviolet light (u.v.). The majority of filaments which developed after irradiation either originated a microcolony or disintegrated. Post-irradiation incubation with chloramphenicol (CMP) restored the ability of some of the bacteria to originate viable colonies by suppressing filament formation, particularly in the case of normal thymine-containing bacteria. CMP also prevented the disintegration of filaments, provided it was applied before they reached a critical length. This was particularly evident in 5-BU-containing bacteria. It is suggested that incubation with CMP may allow wall synthesis to proceed in the absence of protein synthesis, thereby enabling thickening of the wall to take place, so that normal division can resume on removal of the CMP.

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1972-05-01
2024-04-26
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References

  1. Adler H. I., Hardigree A. A. 1965; Post irradiation growth, division and recovery in bacteria. Radiation Research 25:92–102
    [Google Scholar]
  2. Alper T. 1955; Bacteriophage as indicator in radiation chemistry. Radiation Research 2:119–134
    [Google Scholar]
  3. Alper T. 1957; Observations on bacterial growth and morphology shortly after irradiation and some remarks on the oxygen effect. In Advances in Radiobiology, Proceedings of the Fifth International Conference on Radiobiology pp. 90–96 De Hevesey G. C., Forssberg A. G., Abbatt J. D. Edited by Edinburgh: Oliver and Boyd;
    [Google Scholar]
  4. Alper T. 1962; Evidence for two resolvable sites of action of radiation on microorganisms. In Abhand-lungen der Deutschen Akademie der Wissenschaften zu Berlin. (Klasse für Medizin)1 pp. 153–161 Berlin: Akademie-Verlag;
    [Google Scholar]
  5. Alper T. 1963; Lethal mutations and cell death. Physics in Medicine and Biology 8:365–385
    [Google Scholar]
  6. Alper T., Gillies N. E. 1958; Restoration of Escherichia coli strain b after irradiation: its dependence on sub-optimal growth conditions. Journal of General Microbiology 18:461–472
    [Google Scholar]
  7. Alper T., Gillies N. E. 1960; The relationship between growth and survival after irradiation of Escherichia coli strain b and two resistant mutants. Journal of General Microbiology 22:113–128
    [Google Scholar]
  8. Anderson E. H. 1951; Heat reactivation of ultraviolet inactivated bacteria. Journal of Bacteriology 61:389–394
    [Google Scholar]
  9. Bayer M. E., Anderson T. F. 1965; The surface structure of Escherichia coli. Proceedings of the National Academy of sciences of the United States of America 54:1592–1599
    [Google Scholar]
  10. Bazill G. W. 1967; Lethal unbalanced growth in bacteria. Nature; London: 216346–349
    [Google Scholar]
  11. Buzzell A. 1956; The relationships between ultraviolet irradiation and the thermal reactivation of Escherichia coli B. Archives of Biochemistry and Biophysics 62:97–108
    [Google Scholar]
  12. Chung K. L. 1967; Cell wall growth of Bacillus cereus in the presence of chloramphenicol. Canadian Journal of Microbiology 13:341–350
    [Google Scholar]
  13. Gates F. 1933; The reaction of individual bacterial to irradiation with ultraviolet light. science; New York: 77350
    [Google Scholar]
  14. Gillies N. E. 1961; The use of auxotrophic mutants to study restoration in Escherichia coli bbafter ultraviolet irradiation. International Journal of Radiation Biology 3:379–387
    [Google Scholar]
  15. Gillies N. E. 1966; Restoration of 5-bromouracil sensitized Escherichia coli strain b after exposure to ionizing or ultraviolet radiation. Journal of General Microbiology 45:97–111
    [Google Scholar]
  16. Gillies N. E., Alper T. 1959; Reduction in the lethal effects of radiations on Escherichia coli b by treatment with chloramphenicol. Nature; London: 183237–238
    [Google Scholar]
  17. Greer S. 1960; Studies on ultraviolet irradiation of Escherichia coli containing 5-bromouracil in its DNA. Journal of General Microbiology 22:618–634
    [Google Scholar]
  18. Hancock R., Park J. T. 1958; Cell wall synthesis by Staphylococcus aureus in the presence of chloramphenicol. Nature; London: 1811050–1052
    [Google Scholar]
  19. Kantor G. C., Deering R. A. 1966; Ultraviolet radiation studies on filamentous Escherichia coli B. Journal of Bacteriology 92:1062–1069
    [Google Scholar]
  20. Kaplan H. S., Smith K. C., Tomlin P. A. 1962; Effect of halogenated pyrimidines on radiosensitivity of E. coli. Radiation Research 16:98–113
    [Google Scholar]
  21. Mandelstam J., Rogers H. J. 1958; Chloramphenicol-resistant incorporation of amino acids into staphylococci and cell wall synthesis. Nature; London: 181956–957
    [Google Scholar]
  22. Marshall N. J., Gillies N. E. 1972; Chloramphenicol rescue of γ-irradiated Ion and exrA mutants of Escherichia coli. Mutation Research 14:13–21
    [Google Scholar]
  23. Roberts R. B., Aldous E. 1949; Recovery from ultraviolet irradiation in Escherichia coli. Journal of Bacteriology 57:363–375
    [Google Scholar]
  24. Shaw M. K. 1968; Formation of filaments and synthesis of macromolecules at temperatures below the minimum for growth of Escherichia coli. Journal of Bacteriology 95:221–230
    [Google Scholar]
  25. Van De Putte P., Westenbroek C., Rorsch A. 1963; The relationship between gene-controlled radiation resistance and filament formation in Escherichia coli. Biochimica et biophysica acta 76:247–256
    [Google Scholar]
  26. Weinbaum G. 1966; Characteristics of cell walls from morphological variants of Escherichia coli. Journal of General Microbiology 42:83–92
    [Google Scholar]
  27. Witkin E. M. 1947; Genetics of resistance to radiation in Escherichia coli. Genetics 32:221–248
    [Google Scholar]
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