1887

Abstract

SUMMARY: Three temperature-sensitive mutants of have been isolated which, unlike the wild-type, are unable to synthesize DNA at 39°. Synthesis of DNA stops immediately the bacteria are raised to the restrictive temperature. The mutants can be transformed normally for single genetic markers and can regain wild-type temperature resistance on incubation with DNA from wild-type at a frequency associated with single or very closely linked markers. Each mutant can also be transformed to wild-type temperature resistance with DNA from the other temperature-sensitive mutants. All three mutants are resistant to the lethal action of ionizing and ultraviolet radiations and at 39° are able to carry out all or most of the DNA repair functions associated with excision repair, but at a reduced rate compared with wild-type. The rate of excision at 39° of u.v.-induced thymine-containing pyrimidine dimers is four to five times slower in the mutants than in the wild-type.

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/content/journal/micro/10.1099/00221287-70-3-399
1972-05-01
2024-04-27
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References

  1. Anderson A. W., Nordan H. C., Canin R. F., Parrish G., Duggan D. 1956; Studies on a radioresistant micrococcus. Isolation, morphology, cultural characteristics, and resistance to gamma radiation. Food Technology 10:575–578
    [Google Scholar]
  2. Boling M. E., Setlow J. K. 1966; The resistance of Micrococcus radiodurans to ultraviolet radiation. III. A repair mechanism. Biochimica et biophysica acta 123:26–33
    [Google Scholar]
  3. Bollum F. J. 1959; Thermal conversion of non-pairing deoxyribonucleic acid to primer. Journal of Biological Chemistry 234:2733–2744
    [Google Scholar]
  4. Bonhoeffer F., Schaller H. 1965; A method for the selective enrichment of mutants based on the high UV sensitivity of DNA containing 5-bromouracil. Biochemical and Biophysical Research Communications 20:93–97
    [Google Scholar]
  5. Couch J., Hanawalt P. C. 1967; DNA repair replication in temperature-sensitive DNA synthesis deficient bacteria. Biochemical and Biophysical Research Communications 29:779–784
    [Google Scholar]
  6. Duggan D. E., Anderson A. W., Elliker P. R., Cain R. F. 1959; Ultraviolet exposure studies on a gamma radiation resistant micrococcus isolated from food. Food Research 24:376–382
    [Google Scholar]
  7. Hanawalt P. C., Haynes R. H. 1965; Repair replication of DNA in bacteria: irrelevance of chemical nature of base defect. Biochemical and Biophysical Research Communications 19:462–467
    [Google Scholar]
  8. MaaløE O., Hanawalt P. C. 1961; Thymine deficiency and the normal DNA replication cycle. Journal of Molecular Biology 3:144–155
    [Google Scholar]
  9. Moseley B. E. B. 1967; The isolation and some properties of radiation-sensitive mutants of Micrococcus radiodurans. Journal of General Microbiology 49:293–300
    [Google Scholar]
  10. Moseley B. E. B., Mattingly A. 1971; Repair of irradiated transforming deoxyribonucleic acid in wild type and a radiation-sensitive mutant of Micrococcus radiodurans. Journal of Bacteriology 105:976–983
    [Google Scholar]
  11. Pettijohn D., Hanawalt P. 1964; Evidence for repair-replication of ultraviolet damaged DNA in bacteria. Journal of Molecular Biology 9:395–410
    [Google Scholar]
  12. Rupp W. D., Howard-Flanders P. 1968; Discontinuities in the DNA synthesised in an excision defective strain of Escherichia coli following ultraviolet irradiation. Journal of Molecular Biology 31:291–304
    [Google Scholar]
  13. Setlow J. K., Boling M. E. 1965; The resistance of Micrococcus radiodurans to ultraviolet radiation. II. Action spectra for killing, delay in DNA synthesis, and thymine dimerisation. Biochimica et biophysica acta 108:259–265
    [Google Scholar]
  14. Setlow R. B., Carrier W. L., Bollum F. J. 1965; Pyrimidine dimers in UV-irradiated poly dI:dC. Proceedings of the National Academy of sciences of the United States of America 53:1111–1118
    [Google Scholar]
  15. Swenson P. A., Setlow R. B. 1966; Effect of ultraviolet radiation on macromolecular synthesis in Escherichia coli. Journal of Molecular Biology 15:201–219
    [Google Scholar]
  16. Witkin E. M. 1969; Ultraviolet mutation and DNA repair. Annual Review of Microbiology 23:487–514
    [Google Scholar]
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