SUMMARY: Most, though not all, freshly isolated strains of contain haemagglutinin. When organisms are grown on solid medium the haemagglutinin is associated with the cell. In liquid cultures the haemagglutinin in the early stages of growth is also associated with the cell but later diffuses into the medium.

Haemagglutinin can be extracted by sodium chloride or sodium acetate from grown on solid medium. It can be precipitated from the extracts by methanol in the cold and resuspended in phosphate buffer. Precipitation by methanol gives a considerable degree of purification.

Haemagglutinin deteriorates rapidly on storage even at low temperatures. The rate of deterioration is less with solutions in 50% glycerol. Fowl red blood cells or stromata absorb the haemagglutinin but not the toxin. The sodium chloride extract also contains agglutinogen but the sodium acetate extract does not.

Antihaemagglutinin can be prepared by immunizing rabbits with haemagglutinin either in the form of extracts or bacterial suspensions. The most potent antisera are obtained using as antigen red blood cells saturated with haemagglutinin.

Mice infected either intracerebrally or intranasally with cannot be protected either by active immunization with haemagglutinin or by passive immunization with antihaemagglutinin.


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