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Volume 7, Issue 1-2

The Importance of the Temperature during the Early Hours of Incubation of Agar Plates in Assays Free

Abstract

With a note by K. E. COOPER on the theory of inhibition zones

SUMMARY: The time taken for agar plates to reach incubator temperature under different conditions was determined by thermocouple measurements. From 1 to 5 hr. was taken to reach within 1 of the incubator temperature, according to their mass, position, stacking and original temperature. Delay in heating slowed the growth of organisms and produced larger inhibition zones due to diffusing antiseptics. This leads to errors in sensitivity and thus in assay work unless technique is standardized to overcome these differences. Deviations from the calculations resulting from the formula of Cooper & Woodman (1946) can be overcome by such standardization.

An appendix discusses the theory of inhibition zones and compares the formulae proposed by Cooper & Woodman (1946) , by Vesterdal (1947) and by Mitchison & Spicer (1949) .

  • Received:
  • Published Online:
© Society for General Microbiology 1952
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1952-08-01
2024-04-26
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References

  1. Cooper K.E., Gillespie W. 1952; The influence of temperature on streptomycin inhibition zones in agar cultures. J. gen. Microbiol. 7:1
     [Google Scholar]
  2. Cooper K.E., Woodman D. 1946; The diffusion of antiseptics through agar gels, with special reference to the agar cup assay method of estimating the activity of penicillin. J. Path. Bact. 58:75
     [Google Scholar]
  3. Dubos R.J. 1945 The Bacterial Cell pp 137–43 Harvard University Press.:
     [Google Scholar]
  4. Mitchison D.A., Spicer C.G. 1949; A method of estimating streptomycin in serum and other body fluids by diffusion through agar enclosed in glass tubes. J. gen. Microbiol. 3:184
     [Google Scholar]
  5. Schmidt W.H., Moyer A.J. 1944; Penicillin. I. Method of assay. J. Bact. 47:199
     [Google Scholar]
  6. Vesterdal J. 1947; Studies on the inhibition zones observed in the agar cup method for penicillin assay. Acta path, microbiol. scand. 24:272
     [Google Scholar]
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The Importance of the Temperature during the Early Hours of Incubation of Agar Plates in Assays
Microbiology 7, 8 (1952); https://doi.org/10.1099/00221287-7-1-2-8
/content/journal/micro/10.1099/00221287-7-1-2-8
/content/journal/micro/10.1099/00221287-7-1-2-8
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