1887

Abstract

SUMMARY: Mutants of resistant to the antifungal antibiotics, Naramycin or Trichomycin, were isolated after treatment with nitrosoguanidine. When two strains differing in pigmentation and drug resistance were cultured together, amoebae resistant to both drugs appeared in underwater cultures and in liquid shake cultures. Such heterozygotes did not appear during the growth period, but only after cultures reached stationary phase. The appearance of the heterozygotes seems to have some relationship to the state of cells. Studies on the ploidy and the resistance of the progeny of these heterozygous isolates revealed that: (1) diplophase of the original heterozygote is probably transient, (2) all the progeny observed are mononucleate, (3) the ploidy of each progeny settles in a stable state of aneuploidy (between the haploid and the diploid), (4) genetic segregants for drug resistance and pigmentation appear among the progeny in serial subcultures, (5) preferential chromosome elimination occurs during the process of chromosomal reduction. A mechanism of recombination in is discussed in relation to the parasexual cycle.

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1971-08-01
2021-10-16
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References

  1. Adelberg E. A., Mandel M., Chein G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N’-nitro-N-nitrosoguanidine in Escherichia coli k 12. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Bonner J. T. 1947; Evidence for formation of cell aggregation by chemotaxis in the development of Dictyostelium discoideum. Journal of Experimental Zoology 106:1–26
    [Google Scholar]
  3. Bonner J. T. 1967 The Cellular Slime Molds, 2nd edn. pp. 53–55 Princeton, New Jersey: Princeton University Press;
    [Google Scholar]
  4. Bonner J. T., Frascella E. G. 1952; Mitotic activity in relation to differentiation in the slime mould Dictyostelium discoideum. Journal of Experimental Zoology 121:561–571
    [Google Scholar]
  5. Cotter D. A., Raper K. B. 1966; Spore germination in D. discoideum.. Proceedings of the National Academy of Sciences of the United States of America 56:880–887
    [Google Scholar]
  6. Fell H. B., Hughes A. F. 1949; Mitosis in the mouse: a study of living and fixed cells in tissue culture. Quarterly Journal of Microscopical Science 90:355–381
    [Google Scholar]
  7. Fukui Y. 1969 Mutagenesis of drug resistants and isolation of genetic recombinants in the cellular slime mould Dictyostelium discoideum (In Japanese.) Master’s Thesis, Osaka University;
    [Google Scholar]
  8. Hastie A. C. 1967; Mitotic recombination in conidiophores of VerticilHum albo-atrum. Nature, London 214:249–252
    [Google Scholar]
  9. Huffman D. M. 1967; The role of engulfment in the Dictyosteliaceae. Journal of Protozoology 14:762–764
    [Google Scholar]
  10. Huffman D., Kahn A., Olive L. 1962; Anastomosis and cell fusion in Dictyostelium. Proceedings of the National Academy of Sciences of the United States of America 48:1160–1163
    [Google Scholar]
  11. Huffman D. M., Olive L. S. 1964; Engulfment and anastomosis in the cellular slime moulds (Acrasiales). American Journal of Botany 51:465–471
    [Google Scholar]
  12. Käffer E. 1960; High frequency of spontaneous and induced segregation in Aspergillus nidulans. Nature, London 186:619–620
    [Google Scholar]
  13. Loomis W. F. 1969; Temperature-sensitive mutants of Dictyostelium discoideum. Journal of Bacteriology 99:65–69
    [Google Scholar]
  14. Loomis W. F., Ashworth J. M. 1968; Plaque-size mutants of the cellular slime mould Dictyostelium discoideum. Journal of General Microbiology 53:181–186
    [Google Scholar]
  15. Olive L. S. 1963; The question of sexuality in cellular slime moulds. Bulletin of the Torrey Botanical Club 90:144–153
    [Google Scholar]
  16. Pontecorvo G. 1956; The parasexual cycle in fungi. Annual Review of Microbiology 10:393–400
    [Google Scholar]
  17. Pontecorvo G. 1969; Genetic analysis of ’somatic’ cells in filamentous fungi. Heterospecific Genome Interaction pp. 1–8 Edited by Defendi V. The Wistar Institute Symposium Monograph, no. 9, The Wistar Institute Press;
    [Google Scholar]
  18. Pontecorvo G., Roper J. A. 1952; Genetic analysis without sexual reproduction by means of polyploidy in Aspergillus nidulans. Journal of General Microbiology 6:vii–viii
    [Google Scholar]
  19. Raper K. B. 1935; Dictyostelium discoideum, a new species of slime mould from decaying forest leaves. Journal of Agricultural Research 50:135–198
    [Google Scholar]
  20. Raper K. B. 1951; Isolation, cultivation, and conservation of slime moulds. Quarterly Review of Biology 26:169–190
    [Google Scholar]
  21. Ross I. K. 1960; Studies on diploid strains of Dictyostelium discoideum. American Journal of Botany 47:54–59
    [Google Scholar]
  22. Sackin K. J., Ashworth J. M. 1969; An analysis of the distribution of volumes amongst spores of the cellular slime mould Dictyostelium discoideum. Journal of General Microbiology 59:275–284
    [Google Scholar]
  23. Sinha U., Ashworth J. M. 1969; Evidence for the existence of elements of a parasexual cycle in the cellular slime mould Dictyostelium discoideum. Proceedings of the Royal Society of London B, 173:531–540
    [Google Scholar]
  24. Sussman M. 1956; The biology of the cellular slime moulds. Annual Review of Microbiology 10:21–50
    [Google Scholar]
  25. Sussman M. 1964; Isolation of diploid strains of Dictyostelium discoideum from haploid populations. Nature, London 201:216
    [Google Scholar]
  26. Sussman M., Sussman R. R. 1962; Ploidal inheritance in Dictyostelium discoideum: stable haploid, stable diploid and metastable strains. Journal of General Microbiology 28:417–430
    [Google Scholar]
  27. Sussman R. R., Sussman M. 1963; Ploidal inheritance in the slime mould Dictyostelium discoideum: haploidization and genetic segregation of diploid strains. Journal of General Microbiology 30:349–355
    [Google Scholar]
  28. Weiss M. C., Green H. 1967; Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proceedings of the National Academy of Sciences of the United States of America 58:1104–1111
    [Google Scholar]
  29. Wilson C. M. 1952; Sexuality in the Acrasiales. Proceedings of the National Academy of Sciences of the United States of America 38:659–662
    [Google Scholar]
  30. Wilson C. M. 1953; Cytological study of the life cycle of Dictyostelium discoideum. American Journal of Botany 40:714–718
    [Google Scholar]
  31. Wilson C. M., Ross I. K. 1957; Further cytological study in the Acrasiales. American Journal of Botany 44:345–350
    [Google Scholar]
  32. Yanagisawa K., Loomis W. F. Jun., Sussman M. 1967; Developmental regulation of the enzyme UDP-galactose polysaccharide transferase. Experimental Cell Research 46:328–334
    [Google Scholar]
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