1887

Abstract

SUMMARY: The serological relationships of 26 strains of rumen Bacteroides species comprising and were examined using precipitation, agglutination and fluorescent antibody techniques. With precipitation techniques two types of acid-soluble thermostable antigens were detected, a strain-specific antigen and an antigen demonstrating wider serological relationships. With and this latter antigen was species-specific. With , although eight out of 12 strains possessed the same species-specific antigen, there was evidence of two other serological groups within the species. Agglutination tests detected only the strain-specific antigens. Fluorescent antibody tests detected species-specific antigens of and but with only the strain-specific antigens could be demonstrated. Preliminary work with indicated that both kinds of antigen were polysaccharides. Eleven out of 20 freshly isolated strains of reacted with the antiserum. When 30 strains of eight other species of Bacteroides were tested against the species-specific antiserum of the different rumen species of Bacteroides, four strains of and one strain of were found to have a common antigen with that of , and another strain of had a common antigen with another strain. No other cross-reactions were observed.

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1971-08-01
2021-08-03
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References

  1. De Araujo W. C., Varah E., Mergenhagen S. E. 1963; Immunochemical analysis of human oral strains of Fusobacterium and Leptotrichia. Journal of Bacteriology 86:837–844
    [Google Scholar]
  2. Barber C., Vladoianu I. R., Dimache G. 1966; Contributions to the study of Salmonella Immunological specificity of proteins separated from Salmonella typhi.. Immunology 11:287–296
    [Google Scholar]
  3. Barnes E. M., Goldberg H. S. 1968; The relationships of bacteria within the family Bacteroidaceae as shown by numerical taxonomy. Journal of General Microbiology 51:313–324
    [Google Scholar]
  4. Beerens H. 1970; Report of the International Committee on Nomenclature of Bacteria, Taxonomic Subcommittee for Gram-negative anaerobic rods. International Journal of Systematic Bacteriology 20:297–300
    [Google Scholar]
  5. Blackburn T. H. 1968; Protease production by Bacteroides amylophilus strain h1 8. Journal of General Microbiology 53:27–36
    [Google Scholar]
  6. Boivin A., Mesrobeanu L. 1933; Recherches sur les antigenes somatiques et sur les endotoxines des bactéries. Considerations générales et expose des techniques utilisées. Reviews of Immunology 1:553–569
    [Google Scholar]
  7. Bryant M. P. 1959; Bacterial species of the rumen. Bacteriological Reviews 23:125–153
    [Google Scholar]
  8. Bryant M. P., Small N., Bouma C., Chu H. 1958; Bacteroides ruminicola n.sp and Succinimonas amylolytica, the new genus and species. Species of succinic acid-producing anaerobic bacteria of the bovine rumen. Journal of Bacteriology 76:15–23
    [Google Scholar]
  9. Caldwell D. R., Bryant M. P. 1966; Medium without rumen fluid for non-selective enumeration and isolation of rumen bacteria. Applied Microbiology 14:794–801
    [Google Scholar]
  10. Courant P. R., Gibbons R. J. 1967; Biochemical and immunological heterogeneity of Bacteroides melaninogenicus. Archives of Oral Biology 12:1605–1613
    [Google Scholar]
  11. Freeman G. G. 1942; The preparation and properties of a specific polysaccharide from Bact typhosum Ty 2. Biochemical Journal 36:340–355
    [Google Scholar]
  12. Gell P. G. H., Coombs P. R. A. 1968 Clinical Aspects of Immunology. Oxford & Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  13. Hamlin L. J., Hungate R. E. 1956; Culture and physiology of a starch-digesting bacterium (Bacteroides amylophilus n.sp.) from the bovine rumen. Journal of Bacteriology 72:548–554
    [Google Scholar]
  14. Hobson P. N., Mann S. O., Smith W. 1962; Serological tests of a relationship between rumen selenomonads in vitro and in vivo. Journal of General Microbiology 29:265–270
    [Google Scholar]
  15. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bacteriological Reviews 14:1–49
    [Google Scholar]
  16. Hungate R. E. 1966 The Rumen and Its Microbes New York & London: Academic Press;
    [Google Scholar]
  17. Jarvis B. D. W. 1967; Antigenic relations of cellulolytic cocci in the sheep rumen. Journal of General Microbiology 47:309–319
    [Google Scholar]
  18. Jones D., Shattock P. M. F. 1960; The location of the group antigen of group D Streptococcus. Journal of General Microbiology 23:335–343
    [Google Scholar]
  19. Lancefield R. C. 1933; A serological differentiation of human and other groups of hemolytic streptococci. Journal of Experimental Medicine 57:571–595
    [Google Scholar]
  20. Loesche W. J., Socransky S. S., Gibbons R. J. 1964; Bacteroides oralis, proposed new species isolated from the oral cavity of man. Journal of Bacteriology 88:1329–1337
    [Google Scholar]
  21. Mansi W. 1957; The study of some viruses by the plate gel diffusion precipitin test. Journal of Comparative Pathology and Therapeutics 67:297–303
    [Google Scholar]
  22. Reddy C. A., Bryant M. P. 1967; DNA base composition and cytochromes in certain species of the genus Bacteroides. Bacteriological Proceedings p. 40
    [Google Scholar]
  23. Reinhold L. 1965; Studien zur antigenen Struktur an Stämmen aus der Familie der Bacteroidaceen mittels Präzipitation in Agargel. Ernährungsforschung. Internationales Mikrookologisches Symposium 10:498–502
    [Google Scholar]
  24. Ribi E., Kelsey C. M., Perrine T. D. 1959; Endotoxic and antigenic fractions from the cell wall of Salmonella enter it idis. Methods for separation and some biologic activities. Journal of Immunology 82:75–84
    [Google Scholar]
  25. Scheidegger J. J. 1955; A micromethod of immunoelectrophoresis. International Archives of Allergy and Applied Immunology 7:103–110
    [Google Scholar]
  26. Sharpe M. E., Latham M. J., Reiter B. 1969; The occurrence of natural antibodies to rumen bacteria. Journal of General Microbiology 56:353–364
    [Google Scholar]
  27. Sonnenwirth A. C. 1960 A Study of Certain Gram-negative, non-sporulating Anaerobic Bacteria Indigenous to Man, with Special Reference to Their Classification by Serological Means Thesis, Washington University;
    [Google Scholar]
  28. Sonnenwirth A. C. 1970; Serological comparison of Bacteroidaceae strains by indirect bacterial haemagglutination. International Congress for Microbiology 10:193
    [Google Scholar]
  29. Tracey O. 1969; Pigment production in Bacteroides. Journal of Medical Microbiology 2:309–315
    [Google Scholar]
  30. Virginia Polytechnic Institute Anaerobe Laboratory 1970 Outline of clinical methods in anaerobic bacteriology Blacksburg, Virginia: VPI;
    [Google Scholar]
  31. Werner H. 1969; Das serologische Verhalten von Stämmen der Species Bacteroides convexus, B thetaiotaomicron, B. vulgatus and B. distasonis. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, (Abt. Originate) 210:192–201
    [Google Scholar]
  32. Wolin M. J. 1966; Lysis of Vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. Journal of Bacteriology 91:1781–1786
    [Google Scholar]
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