1887

Abstract

Homogenates of suspensions were prepared aftergrowth in the absence and presence of chloramphenicol (500 μg./ml.). Sedimentation of organelles through gradients of aqueous sucrose solutions was studied by means of a zonal centrifuge. Mitochondria (marker enzyme, malate dehydrogenase) from organisms grown with chloramphenicol sediment more slowly (median sedimentation coefficient in water at 20° = 10,600 ) than those from normal cells (median sedimentation coefficient in water at 20° = 90,000 , which confirms that the former are of smaller volume than normal mitochondria. After high-speed zonal centrifugation both mitochondria (marker enzymes, NADH and NADPH-cytochrome oxidoreductases) attain similar equilibrium densities (median density = 1·21 to 1·22). The buoyant density (median = 1·23) of peroxisomes (marker enzyme, catalase) is not altered by the inclusion of chloramphenicol in the growth medium, but the proportion of non-sedimentable catalase is increased. Chloramphenicol-inhibited growth also leads to a change in the distribution of lysosomes (marker enzymes, acid -nitrophenolphosphatase, and N-acetyl-β(D)-glucosaminidase); a marked reduction in the population at = 1·15 to 1·20 is accompanied by the appearance at = 1·09 to 1·15 of a new class of organelles containing acid hydrolases.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-67-2-161
1971-08-01
2019-10-16
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-67-2-161
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error