1887

Abstract

SUMMARY: The utilization and translocation of C by micro-organisms, especially fungi, was shown to occur during the decomposition of labelled plant residues in agar cultures and in soil. Utilization was demonstrated by the evolution of CO and by autoradiograms that showed C within micro-organisms. Translocation was indicated by labelled structures occurring on coverslips at a distance from the substrate, and by the labelling of sclerotia which had accumulated C preferentially nearly 1 cm. from the plant residues. sp. and , shown to be cellulolytic on cotton cellulose agar, were similarly active in agar and soil cultures containing radioactive plant residues. Labelled structures of spp., spp., spp. and fungi with non-sporing dark mycelia were detected in non-sterile soil during the later stages of the decomposition of the labelled plant residues when a marked fall in carbon content was occurring. The autoradiographic technique employed here enables organisms that probably act as primary agents in the degradation of plant residues to be distinguished from those that do not.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-66-3-339
1971-06-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/66/3/mic-66-3-339.html?itemId=/content/journal/micro/10.1099/00221287-66-3-339&mimeType=html&fmt=ahah

References

  1. Buller A. H. R. 1933 In Researches on Fungi vol 5 London, New York & Toronto: Longman;
    [Google Scholar]
  2. Catch J. R., Evans E. A. 1960; Rate of formation of atropine in Atropa belladonna plants. Nature, London 188:758–759
    [Google Scholar]
  3. Edelman J., Shibko S. I., Keys A. J. 1959; The role of the scutellum of cereal seedlings in the synthesis and transport of sucrose. Journal of Experimental Botany 10:178–189
    [Google Scholar]
  4. Eggins H. O. W., Pugh G. J. F. 1962; Isolation of cellulose-decomposing fungi from the soil. Nature, London 193:94–95
    [Google Scholar]
  5. Garrett S. D. 1962; Decomposition of cellulose in soil by Rhizoctonia solani Kuhn. Transactions of the British Mycological Society 45:115–120
    [Google Scholar]
  6. Grossbard E. 1958; Autoradiography of fungi through a layer of soil and in agar culture. Nature, London 182:854–856
    [Google Scholar]
  7. Grossbard E. 1959 In Experiments in Progress. Grassland Research Institute, Hurley 13:69–70
    [Google Scholar]
  8. Grossbard E. 1962; Autoradiography of bacteria and Streptomycetaceae by the stripping film technique. Nature, London 193:853–855
    [Google Scholar]
  9. Grossbard E. 1969; A visual record of the decomposition of 14C-labelled fragments of grasses and rye added to soil. Journal of Soil Science 20:38–51
    [Google Scholar]
  10. Grossbard E., Hall D. M. 1962; Plant protein agar, a medium for the isolation and estimation of proteolytic organisms in grassland soils. Nature, London 196:119–120
    [Google Scholar]
  11. Grossbard E., Stranks D. R. 1959; Translocation of cobalt-60 and caesium-137 by fungi in agar and soil cultures. Nature, London 184:310–314
    [Google Scholar]
  12. Hill E. P. 1965; Uptake and translocation.. In The Fungi pp 457–463 Edited by Ainsworth G. C., Sussmann A. S. New York: Academic Press;
    [Google Scholar]
  13. Lucas R. L. 1960; Transport of phosphorus by fungal mycelium. Nature, London 188:763–764
    [Google Scholar]
  14. Paul A. G., Scully N. J., Schwarting A. E. 1960; The biosynthesis of 14C-labelled ergot alkaloids. Journal of the American Pharmaceutical Association—Scientific Edition 49:14–16
    [Google Scholar]
  15. Pelc S. R. 1956; The stripping film technique of autoradiography. International Journal of Applied Radiation and Isotopes 1:172–177
    [Google Scholar]
  16. Pugh G. J. F. 1964; An investigation of soil-borne cellulose-decomposing fungi in Greece. Annales de l’Institut Phytopathologique Benaki N.S. 7, 19–27
    [Google Scholar]
  17. Rogers A. W. 1967 Techniques of autoradiography pp 246–251 Amsterdam, London & New York: Elsevier;
    [Google Scholar]
  18. Schütte K. H. 1956; Translocation in the fungi. New Phytologist 55:164–182
    [Google Scholar]
  19. Skinner F. A. 1960; The isolation of anaerobic cellulose-decomposing bacteria from soil. Society for General Microbiology 22:539–554
    [Google Scholar]
  20. Wilcoxson R. D., Subbarayudu S. 1968; Translocation to and accumulation of phosphorus-32 in sclerotia of Sclerotium rolfsii. Canadian Journal of Botany 46:85–88
    [Google Scholar]
  21. Wilcoxson R. D., Sudia T. W. 1968; Translocation in fungi. Botanical Review 34:32–51
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-66-3-339
Loading
/content/journal/micro/10.1099/00221287-66-3-339
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error