1887

Abstract

SUMMARY: A ribonuclease found in preparations of streptolysin S, unlike the strepto-coccal nucleases so far described, does not degrade DNA. It is only produced in significant amounts when RNA is present in the medium and is therefore an inducible enzyme. Unlike the previously described streptococcal enzymes it does not require divalent cations for activity. The molecular weight of the enzyme is about 14,000, its isoelectric point is 9.5 and it is highly heat-stable.

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/content/journal/micro/10.1099/00221287-65-2-193
1971-02-01
2024-04-26
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References

  1. Andrews P. 1964; Estimation of the molecular weights of proteins by sephadex gel filtration.. Biochemical Journal 91:222–233
    [Google Scholar]
  2. Barnard E. A. 1969; Ribonucleases.. Annual Review of Biochemistry677–732
    [Google Scholar]
  3. Bernheimer A. W. 1949; Formation of a bacterial toxin (streptolysin S) by resting cells.. Journal of Experimental Medicine 90:373–392
    [Google Scholar]
  4. Bernheimer A. W., Ruffier N. K. 1951; Elaboration of deoxyribonuclease by streptococci in the resting state and inhibition of the enzyme by a substance extractable from the cocci.. Journal of Experimental Medicine 93:399–413
    [Google Scholar]
  5. Glitz D. G., Dekker C. A. 1964; Studies on a ribonuclease from Ustilago sphaerogena. I. Purification and properties of the enzyme.. Biochemistry 3:1391–1399
    [Google Scholar]
  6. Gray E. D., Yasmineh W. G. 1968; Streptococcal nucleases. IV. Some properties and specificities of the ribonuclease action of the B and D enzymes.. Biochemistry 7:98–105
    [Google Scholar]
  7. Koerner J. F., Sinsheimer R. L. 1957; A deoxyribonuclease from calf spleen.II. Mode of action.. Journal of Biological Chemistry 228:1049–1062
    [Google Scholar]
  8. Krumwiede E. 1954; Studies on a lipoproteinase of group A streptococci.. Journal of Experimental Medicine 100:629–639
    [Google Scholar]
  9. McCarty M. 1946; Purification and properties of deoxyribonuclease isolated from beef pancreas.. Journal of General Physiology 29:123–139
    [Google Scholar]
  10. McCarty M. 1948; The occurrence of nucleases in culture filtrates of group A haemolytic streptococci.. Journal of Experimental Medicine 88:181–188
    [Google Scholar]
  11. McDonald M. R. 1955; Ribonucleases.. Methods in Enzymology 2 New York: Academic Press.;
    [Google Scholar]
  12. Nishimura S., Nomura M. 1959; Ribonuclease of Bacillus subtilis. . Journal of Biochemistry, Tokyo 46:161–167
    [Google Scholar]
  13. Takai N., Uchida T., Egami F. 1967; Ribonuclease, phosphodiesterases and phosphomonoesterase of Neurospora crassa in various culture conditions.. Journal of the Japanese Biochemical Society (Seikagaku) 39:285–290
    [Google Scholar]
  14. Wannamaker L. W. 1958; The differentiation of three distinct deoxyribonucleases of group A streptococci.. Journal of Experimental Medicine 107:797–812
    [Google Scholar]
  15. Wannamaker L. W. 1962; Characterization of a fourth deoxyribonuclease of group A streptococci.. Federation Proceedings. Federation of American Societies for Experimental Biology 21:231
    [Google Scholar]
  16. Wannamaker L. W. 1964; Streptococcal deoxyribonucleases.. The Streptococcus, Rheumatic Fever and Glomerulonephritis Edited by Uhr J. W. Baltimore, Maryland: Williams and Wilkins Company.;
    [Google Scholar]
  17. Yanagida M., Uchida T., Egami F. 1964; Culture of Ustilago zeae with RNA or poly U as phosphorus source.. Journal of the Agricultural Society of Japan 38:531–535
    [Google Scholar]
  18. Yasmineh W. G., Gray E. D., Wannamaker L. W. 1968; Streptococcal nucleases. III. Kinetics of action and inhibition by transfer ribonucleic acid.. Biochemistry 7:91–97
    [Google Scholar]
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