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SUMMARY: At low concentrations (0·03 to 3 μg./109 cells), purified prymnesium toxin lysed penicillin and lysozyme-EDTA spheroplasts of Escherichia coli and Pseudomonas fluorescens and protoplasts of Micrococcus lysodeikticus and Bacillus subtilis. Intact E. coli and P. fluorescens were unaffected by the toxin (3·3 μg./ml.), but E. coli b was lysed by prymnesium toxin in the presence of EDTA. The activities of selected detergents against spheroplasts and EDTA-treated E. coli b were compared with the action of prymnesium toxin.
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