1887

Abstract

SUMMARY: Crude extracts of 146 strains of different species of Lactobacillus have been submitted to starch gel electrophoresis and the gels developed specifically for lactic dehydrogenases, D or L. NAD-dependent or NAD-independent. Each species contained at least one NAD-dependent lactic dehydro-genase specific for D(-) or for L(+) lactic acid; some species contained both enzymes. For each species there was a strict correlation between the stereo-specificity of the NAD-dependent lactic dehydrogenases found in extracts and the type of lactic acid produced during growth, i.e. D(-), L(+) or DL. The L-lactic dehydrogenases (NAD-dependent) of and were not reversible and consequently could not be detected after electrophoresis. Only those NAD-independent lactic dehydrogenases able to reduce phenazine methosulphate could be detected by the method of development used. Such enzymes were found in extracts of some homo-fermentative lactobacilli.

The number and the nature of the lactic dehydrogenases and their respective electrophoretic mobilities are constant within a species. Therefore the electrophoretic migration of lactic dehydrogenases of lactobacilli is a useful taxonomic marker for species differentiation.

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/content/journal/micro/10.1099/00221287-62-2-223
1970-08-01
2019-10-19
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-62-2-223
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