1887

Abstract

Surmmary

The ability to produce opacity in horse serum is a characteristic of certain M types of group A streptococci. The types that produce the opacity factor (O factor) are generally those for which it is difficult to produce good anti-M sera. M-positive (M+) and true M-negative (M − ) variants of strains that belong to serotypes in which the serum opacity reaction (s.o.r.) is positive both possess the O factor, but there is a difference in binding of the factor to other cellular components in the two variants. The O factor is closely bound to the wall-membrane fraction of M − cells, whereas in M+ cells it is easily extracted by Lancefield’s method or 1 % sodium deoxycholate.

It is detectable in broth culture supernatants, in the cytoplasm and in the areas surrounding colonies in pour plates of M+ but not of M − cultures.

The O factor is poorly antigenic, but when it is possible to obtain a good antiserum the inhibitory action is type specific. The O factor produced by several M types appears to be inhibited by normal rabbit serum.

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/content/journal/micro/10.1099/00221287-61-3-343
1970-06-01
2021-08-05
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References

  1. Gooder H. 1961; Association of a serum opacity reaction with serological type in Streptococcus pyogenes . Journal of General Microbiology 25:347–352
    [Google Scholar]
  2. Gooder H., Maxted W. R. 1958; Protoplasts of group A β-haemolytic streptococci. Nature; London: 182808–809
    [Google Scholar]
  3. Griffith F. 1934; The serological classification of Streptococcus pyogenes . Journal of Hygiene, Cambridge 34:542–584
    [Google Scholar]
  4. Köhler W. 1963; Serum-Opazitäts-Reaktion (SOR) und die Bildung des M-antigens durchStreptococcus pyogenes . Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene Abteilung 1 189:275–281
    [Google Scholar]
  5. Krumwiede E. 1954; Studies on a lipoproteinase of group A streptococci. Journal of Experimental Medicine 100:629–639
    [Google Scholar]
  6. Lancefield R. C., Todd E. W. 1928; Antigenic differences between matt hemolytic streptococci and their glossy variants. Journal of Experimental Medicine 48:769–790
    [Google Scholar]
  7. Mackie & McCartney’s Handbook of Bacteriology 1960, 10th edn. p. 190 Edinburgh & London: E. & S Livingstone;
  8. Maxted W. R. 1955; The influence of bacteriophage on Streptococcus pyogenes . Journal of General Microbiology 12:484–495
    [Google Scholar]
  9. Maxted W. R., Valkenburg H. A. 1969; Variation in the M antigen of group A streptococci. Journal of Medical Microbiology 2:199–210
    [Google Scholar]
  10. Maxted W. R., Valkenburg H. A. 1970; Variations in the M antigen of group A streptococci. Proceedings of the Fourth International Symposium on Streptococcus pyogenes, Jena, East Germany, 29 September-3 October 1969. (In preparation.)
    [Google Scholar]
  11. Michel M. F., Gooder H. 1962; Amino acids, amino sugars and sugars present in the cell walls of some strains of Streptococcus pyogenes . Journal of General Microbiology 29:199–205
    [Google Scholar]
  12. Rowen R., Martin J. 1963; Enhancement of cholesterol esterification in serum by an extract of a group A streptococcus. Biochimica et Biophysica Acta 70:396–405
    [Google Scholar]
  13. Swift H. F., Wilson A. T., Lancefield R. C. 1943; Typing group A hemolytic streptococci by M precipitin reactions in capillary pipettes. Journal of Experimental Medicine 78:127–133
    [Google Scholar]
  14. Top F. H. Jun. Wannamaker L. W. 1968a; The serum opacity reaction of Streptococcus pyogenes: Frequency of production of streptococcal lipoproteinase by strains of different serological types and relationship to M protein production. Journal of Hygiene, Cambridge 66:49–58
    [Google Scholar]
  15. Top F. H. Jun. Wannamaker L. W. 1968b; The serum opacity reaction of Streptococcus pyogenes. The demonstration of multiple strain specific lipoproteinase antigens. Journal of Experimental Medicine 127:1013–1034
    [Google Scholar]
  16. Widdowson J. P., Maxted W. R., Grant D. L. 1970; The serum opacity reaction in group A streptococci. Proceedings of the Fourth International Symposium on Streptococcus pyogenes, Jena, East Germany, 29 September-3 October 1969. (In preparation.)
    [Google Scholar]
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